酶联免疫法(ELISA)测定瓶装黄酒中黄曲霉毒素B  

Determination of aflatoxin B1 in bottled Chinese rice wine by enzyme-linked immunosorbent assay( ELISA)

作　　者：吉小凤[1] 陈笑芸[1] 魏巍[1] 

机构地区：[1]浙江省农业科学院农产品质量标准研究所,浙江杭州310021

出　　处：《浙江农业学报》2015年第11期2006-2010,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省国际合作项目(2013C24021)

摘　　要：采用酶联免疫法对瓶装黄酒中黄曲霉毒素B1含量进行测定,并对该方法的线性、灵敏度、回收率和重现性进行验证,同时对如何预防瓶装黄酒中黄曲霉毒素B1污染进行讨论。黄曲霉毒素B1标准品在0.1～2.0μg·L-1范围内线性良好,y=-0.4679x＋0.3132,R2=0.998 2,该方法对黄曲霉毒素B1的最低检出浓度为0.05μg·kg-1,不同浓度添加回收试验所得回收率为90.2%～106.0%,重复性好,精密度为3.5%。通过对26个不同产品特性黄酒样品中黄曲霉毒素B1的测定,发现所有样品均有检出,但黄曲霉毒素B1的含量均小于2.0μg·kg-1。酶联免疫检测方法研究结果表明,该方法测定快速,定量准确,重现性好,可高效率地定量检测大量市售黄酒样品中黄曲霉毒素B1的含量。The present study was conducted by enzyme-linked immunosorbent assay（ ELISA） to determine aflatoxin B1（ AFB1） content in bottled Chinese rice wine. The linearity,detection sensitivity,recovery and repeatability were verified. High correlation coefficient was obtained（ R2= 0. 998 2） within the linear range（ 0. 1 ～ 2. 0 μg·L- 1）.The detection limit for AFB1 content was 0. 05 μg·kg- 1. The average spiked recovery rate was 90. 2% ～ 106. 0%,and the repeatability was 3. 5%. The developed method was applied to 26 bottled Chinese rice wine collected from local markets. AFB1 was found within all collected samples,but the content of AFB1 in each wine was all below 2. 0μg·kg- 1. This method could be widely applied to determine aflatoxin B1 content in a large number of commercially available Chinese rice wine.

关 键 词：酶联免疫法 黄曲霉毒素B1 黄酒 

分 类 号：TS207.3[轻工技术与工程—食品科学]