大豆种子醇溶蛋白的提取及SDS-PAGE分析  

Analysis of the Soybean Seed Gliadia with SDS-PAGE

作　　者：张艳馥[1] 任艳波[1] 沙伟[1] 王雪[1] 宋璐[1] 孙博[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《种子》2015年第11期41-43,共3页Seed

基　　金：黑龙江省教育厅科学技术研究项目(编号:12531755)

摘　　要：以大豆垦丰16种子为实验材料,采用乙二醇、乙醇和异丙醇3种提取试剂,运用正交试验设计提取大豆种子醇溶蛋白,并对不同提取体系所得到的醇溶蛋白进行SDS聚丙烯凝胶电泳分析。结果显示:不同的提取剂提取大豆种子醇溶蛋白的浓度不同;同一提取剂,不同提取条件所提取的大豆种子醇溶蛋白浓度不同。3种提取试剂中乙二醇提取体系种子醇溶蛋白浓度较高。以乙二醇为提取试剂,固液比为1∶13、提取温度为50℃、提取时间为1h、提取剂浓度为50%,上样量为15μL进行大豆种子醇溶蛋白SDS-PAGE效果较好。The soybean seed gliadia wear extracted with different extracting solvents,analysis the soybean seed gliadia by SDS-PAGE.The results showed that the concentration of seed gliadia was different with different extracting solvents;the concentration of seed gliadia was different with same extracting solvents under different condition.the good effect of SDS-PAGE of soybean seed gliadia could be obtained when with the extracting solvents of ethylene glycol,under ratio of solid to liquid 1∶13,temperature 50℃,time 1h,the concentration of extracting solvents was 50%,and 15μL loading sample.

关 键 词：大豆 醇溶蛋白 提取 SDS-PAGE 

分 类 号：S565.1[农业科学—作物学]