食品中大肠菌群快速检测效果评价  

Effect evaluation of rapid detection of coliform group in food

作　　者：李树立[1] 刘坤[1] 张香美[1] 郝秋娟[1] 

机构地区：[1]河北经贸大学生物科学与工程学院,河北石家庄050061

出　　处：《现代预防医学》2015年第23期4265-4267,共3页Modern Preventive Medicine

摘　　要：目的分析复合显色培养基在食品检验中快速测定大肠菌群的作用。方法以大肠菌群标准菌株及其他食源性致病菌标准菌株、85份食品样品为研究对象对象,同时采取国标GB4789.3中对大肠菌群的检测方法以及复合显色培养法进行大肠菌群的检测,比较两者的检测时间﹑准确性等。综合评价该复合显色培养基的效果。结果将标准菌株大肠杆菌、弗氏柠檬酸杆菌、阴沟肠杆菌、阪崎肠杆菌、产气肠杆菌和肺炎克雷伯菌接种在复合显色平板及VRBA平板,均见生长,呈蓝绿色。而福氏志贺菌、副溶血性弧菌和金黄色葡萄球菌非大肠菌群属细菌在复合显色平板上培养18 h后,菌落无生长。检测食品样本的阳性率与国标大肠菌群VRBA平板法差异无统计学意义(P>0.05)。结论复合显色培养基可以快速检测食物中大肠菌群并且准确度高,值得在食品检测中使用。Objective To analyze the effect of complex chromogenic medium for rapid determination of coliform group in food inspection. Methods Coliform standard strains, other food-borne pathogens standard strains and 85 food samples were used as the research object, respectively. The fecal coliform detection method in national standard GB4789.3 and composite chromogenic culture method for fecal coliform were adopted to detect the pathogens. The detection time and accuracy were compared between the two method. A comprehensive evaluation of the effects of the composite chromogenic medium was made. Results Standard bacteria were inoeulated on the composite color fiat and VRBA fiat. E.coli Freund＇s citric acid bacillus, Sewer e.eoli, E. Sakazakii, Enterobaeter aerogenes and Klebsiella pneumonia bacteria were growed on both of them, with the color of blue-green. There were no non-coliform bacteria including Shigella bacteria, Vibrio parahaemolyticus and Staphylococcus anreus on the composite color fiat after 18h training. There was no significant difference between the composite color fiat and VRBA fiat in the detection of coliform bacteria （P〉0.05）. Conclusion Compound ehromogenic medium can quickly detect coliform group with high accuracy in food. It is worthy to be used in the detection of food.

关 键 词：显色培养基 大肠菌群 快速检测效果 

分 类 号：R15[医药卫生—营养与食品卫生学]