Combination of fasudil and celecoxib promotes the recovery of injured spinal cord in rats better than celecoxib or fasudil alone  

作　　者：Xiao-lin Hou Yan Chen Hua Yin Wei-gang Duan 

机构地区：[1]Key Laboratory of Molecular Biology for Sinomedicine, Yunnan Key Laboratory for Enthnomedicine [2]Initiative Team of Microenvironment, Yunnan University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》2015年第11期1836-1840,共5页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China;No.81060109;a grant from the Yunnan Provincial Department of Science&Technology in China;No.2008CD150

摘　　要：Resistance mechanisms of rho-associated kinase（ROCK） inhibitors are associated with the enhanced expression of cyclooxygenase-2（COX-2）. The therapeutic effects of ROCK on nervous system diseases might be enhanced by COX-2 inhibitors. This study investigated the synergistic effect of the combined use of the ROCK inhibitor fasudil and a COX-2 inhibitor celecoxib on spinal cord injury in a rat model established by transecting the right half of the spinal cord at T11. Rat models were orally administrated with celecoxib（20 mg/kg） and/or intramuscularly with fasudil（10 mg/kg） for 2 weeks. Results demonstrated that the combined use of celecoxib and fasudil significantly decreased COX-2 and Rho kinase II expression surrounding the lesion site in rats with spinal cord injury, improved the pathomorphology of the injured spinal cord, and promoted the recovery of motor function. Moreover, the effects of the drug combination were better than celecoxib or fasudil alone. This study demonstrated that the combined use of fasudil and celecoxib synergistically enhanced the functional recovery of injured spinal cord in rats.Resistance mechanisms of rho-associated kinase（ROCK） inhibitors are associated with the enhanced expression of cyclooxygenase-2（COX-2）. The therapeutic effects of ROCK on nervous system diseases might be enhanced by COX-2 inhibitors. This study investigated the synergistic effect of the combined use of the ROCK inhibitor fasudil and a COX-2 inhibitor celecoxib on spinal cord injury in a rat model established by transecting the right half of the spinal cord at T11. Rat models were orally administrated with celecoxib（20 mg/kg） and/or intramuscularly with fasudil（10 mg/kg） for 2 weeks. Results demonstrated that the combined use of celecoxib and fasudil significantly decreased COX-2 and Rho kinase II expression surrounding the lesion site in rats with spinal cord injury, improved the pathomorphology of the injured spinal cord, and promoted the recovery of motor function. Moreover, the effects of the drug combination were better than celecoxib or fasudil alone. This study demonstrated that the combined use of fasudil and celecoxib synergistically enhanced the functional recovery of injured spinal cord in rats.

关 键 词：nerve regeneration Rho kinase FASUDIL CYCLOOXYGENASE-2 CELECOXIB spinal cord injury NSFC grant neural regeneration 

分 类 号：R651.2[医药卫生—外科学]