红树植物桐花树提取物清除亚硝酸盐与阻断亚硝胺合成的体外评价  

Evaluation in vitro of Scavenging Effect on Nitrite and Interdicting Effect on Nitrosamine Synthesis of Extract from Aegiceras corniculatum

作　　者：黄晓冬[1,2] 李裕红[1] 戴聪杰[1] 李元跃 黄晓昆[1] 

机构地区：[1]泉州师范学院近海资源生物技术福建省高校重点实验室,福建泉州362000 [2]福建省海洋渔业资源与生态环境重点实验室,福建集美361000

出　　处：《中国食品学报》2015年第9期15-22,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省自然科学基金项目(2015J01146);福建省海洋渔业资源与生态环境重点实验室开放基金项目(fmfre2014011);福建省教育厅科技项目(JA12278)

摘　　要：在模拟人体胃液的条件下,以亚硝酸盐清除率与亚硝胺合成阻断率为指标,考察桐花树叶乙醇提取物、多酚提取物与总黄酮提取物对亚硝化反应的抑制作用。结果表明:在模拟胃液条件下,桐花树叶乙醇提取物、多酚提取物与总黄酮提取物均能不同程度地清除亚硝酸盐与阻断亚硝胺合成。6 mg/m L桐花树叶醇提取物处理3 h,亚硝酸盐清除率高达90.31%;14 mg/m L桐花树叶醇提取物作用3.5 h,N-亚硝胺阻断率为57.44%。8 mg/m L多酚提取物处理3 h,亚硝酸盐清除率达到100%。对NDMA合成阻断的最适条件:7 mg/m L多酚提取物处理3 h,阻断率可达82.20%。45 mg/m L总黄酮提取物作用5 h,亚硝酸盐清除率达到100%。对NDMA合成阻断的最适条件:35 mg/m L总黄酮提取物处理3.5 h,阻断率为75.40%。总体表现为桐花树多酚提取物的亚硝酸盐清除能力与亚硝胺合成阻断能力强于醇提取物与总黄酮提取物。In mimic gastric juice, the inhibiting effects of ethanol extract, polyphenol extract and favonoids extract from Aegiceras corniculatum leaves on nitrosation reaction were investigated with scavenging ratio on nitrite and interdict- ing ratio on nitrosamine synthesis as the index. The results showed that ethanol extract, polyphenol extract and favonoidsextracts from Aegiceras corniculatum could scavenge nitrite and interdict nitrosamine synthesis to different extents. The scavenging ratio on nitrite of ethanol extract from Aegiceras corniculatum leaves reached up to 90.31% under the condi- tion of 6 mg/mL concentration and 3 h reaction time, the interdicting ratio on nitrosamine synthesis of ethanol extract was 57.44% under the condition of 14 mg/mL concentration and 3.5 h reaction time. The scavenging ratio on nitrite of polyphenol extract from Aegiceras corniculatum leaves reached 100% under the condition of 8 mg/mL concentration and 3h reaction time, and when the optimal condition was 7 mg/mL concentration and 3 h reaction time, the interdicting ratio on nitrosamine synthesis reached 82.20%. The scavenging ratio on nitrite of favonoids extract from Aegiceras corniculatum leaves reached 100% under the condition of 45 mg/mL concentration and 5 h reaction time, its interdicting ratio on ni- trosamine synthesis was 75.40% under the optimal condition of 35 mg/mL concentration and 3.5 h reaction time. Overall performance showed that the scavenging ability on nitrite and interdicting ability on nitrosamine synthesis of polyphenol extract from Aegiceras corniculatum leaves was stronger than ethanol extract and favonoids extract.

关 键 词：桐花树 乙醇提取物 总黄酮 多酚 亚硝酸盐 亚硝胺 

分 类 号：TS201.2[轻工技术与工程—食品科学]