鞘氨醇单胞菌发酵生产韦兰胶的研究  

Study on fermentation of welan gum by Sphingomonas sp.

作　　者：董学前 刘元涛 王伟 张永刚 武琳 吉武科 刘建军 

机构地区：[1]山东省食品发酵工业研究设计院,山东济南250013 [2]阜丰集团有限公司,山东莒南276600

出　　处：《发酵科技通讯》2015年第4期30-33,共4页Bulletin of Fermentation Science and Technology

摘　　要：韦兰胶(welan gum)是由鞘氨醇单胞菌(Sphingomonas paucimobilis)分泌的一种可溶性胞外多糖。利用实验室优化筛选的一株韦兰胶产生菌Sphingomonas sp.511,通过单因素实验,研究了韦兰胶发酵工艺条件,得出韦兰胶适宜的发酵条件为:种龄15 h,接种量10%,装液量80 m L(500 m L摇瓶),初始p H 8.0,摇床转速300 r/min,培养温度32℃,发酵时间72 h,在此实验基础上进行10 L发酵罐的放大试验,韦兰胶产率为25.76 g/L。Welan gum is a soluble exopolysaccharide produced by Sphingomonas sp.The fermentation conditions were optimized by single-factor experiments using a welan gum producing strain Sphingomonas sp.511 screened in our laboratory.The optimal fermentation conditions were as follows：inoculum age 15 h,inoculum volumn 10%（V/V）,medium volumetric ratio 16%（V/V）,initial p H value 8.0,rotate speed 300 r/min,culture temperature 32 ℃ and fermentation period 72 h.The scale-up test was carried out under these conditions and the concentration of welan gum reached 25.76 g/L.

关 键 词：韦兰胶 鞘氨醇单胞菌 发酵工艺 

分 类 号：TQ92[轻工技术与工程—发酵工程]