改良分光光度法测定鸡蛋中四环素  

Determination of tetracyline in eggs by improved spectrophotometry

作　　者：刘月英[1] 郎杰[1] 李琨[1] 

机构地区：[1]河北经贸大学生物科学与工程学院,河北石家庄050061

出　　处：《中国食品卫生杂志》2015年第6期639-642,共4页Chinese Journal of Food Hygiene

摘　　要：目的对鸡蛋中的四环素进行测定,以保证食品安全。方法本试验对分光光度法做了改良,用合适的提取剂提取6个样品中四环素后进行富集,再用分光光度法测定,可提高鸡蛋四环素的检测效果。结果 6个样品的四环素平均含量分别为237.2、183.1、148.0、231.8、219.4、252.3μg/kg,回收率均在98%以上。结论本方法操作简便,设备简单,较适合基层检测部门、养殖企业及没有高效液相色谱设备的单位检测鸡蛋中的四环素。Objective Tetracycline in eggs need to be detected to ensure food safety. Methods Spectrophotometry was improved in the experiment. Tetracycline from 6 different samples was extracted respectively with suitable extraction reagent and enriched, and then determined by spectrophotometry. Results The average contents of tetracycline in the 6 samples were 237.2, 183. 1, 148.0, 231.8, 219. 4 and 252. 3 μg/kg respectively and the recovery was above 98%. Conclusion The method was easy to operate and the equipment was simple. It is suitable for detection of tetracycline in eggs in local inspection departments, poultry farms and units without high performance liquid chromatography.

关 键 词：鸡蛋 四环素残留 分光光度法 改良 抗生素 兽药残留 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.32[医药卫生—公共卫生与预防医学]