在线固相萃取-高效液相色谱法快速检测辣椒粉中的苏丹红  

Rapid determination of sudan dyes in chilli power by online solid phase extraction-high performance liquid chromatography

作　　者：朱群英[1] 朱玉玲[1] 索莉莉[1] 李良[1] 

机构地区：[1]南昌市疾病预防控制中心,江西南昌330038

出　　处：《食品工业科技》2015年第24期67-70,共4页Science and Technology of Food Industry

基　　金：江西省科技计划(20142BBG70032)

摘　　要：建立在线固相萃取-高效液相色谱快速检测辣椒粉中苏丹红染料的方法。样品经乙腈超声提取离心过滤后直接进样,在富集柱上进行纯化和富集,通过阀的切换把富集柱上的被测组分苏丹红转移至分析柱上分离,外标峰面积法定量。四种苏丹红在1~200 ng/m L浓度范围内具有良好的线性关系（r≥0.9992）,检出限为1.0~1.95 ng/g,加标回收率为86.2%~101.6%,相对标准偏差小于6.18%。该方法简单,快速,准确可靠,满足辣椒粉中苏丹红染料的快速检测要求。A novel method was developed for the rapid determination of sudan dyes in chilli power by online solid-phase extraction coupled to high liquid performance chromatography（online SPE-HPLC）. Chilli power was extracted with acetonitrile. Then the extract was centrifuged and filtered. After that,1 m L of extract was injected into online SPE system by autosampler and delivered to the SPE column for purification and enrichment using loading pump. After clean-up step,the online SPE column was switched to the analytical flow path by valve switching and the analytes were back flushed to the analytical column using analytical pump for the further determination. Under the optimized conditions,good linearities were obtained in the range from 1 ng/m L to200 ng/m L for the four sudan dyes,with correlation coefficients above 0.9996. The limits of detection for all the analytes ranged from 1.0 ng/g to 1.95 ng/g. The method recoveries of four analytes at three spiked levels ranged between 86.2% and 101.6%,with relative standard deviations less than 6.18%. The proposed method was simple,rapid,accurate and reliable,and could be applied to the rapid determination of sudan dyes in chilli power.

关 键 词：在线固相萃取 高效液相色谱 苏丹红 快速检测 辣椒粉 

分 类 号：TS201.1[轻工技术与工程—食品科学]