提取方法对类球红细菌辅酶Q10抗氧化活性的影响  

Effect of Extraction Methods on Antioxidant Activities of Coenzyme Q10 from Rhodobacter sphaeroides

作　　者：李祖明[1] 安君[1] 常平[1] 白志辉[2] 

机构地区：[1]北京联合大学应用文理学院,北京100191 [2]中国科学院生态环境研究中心,北京100085

出　　处：《沈阳农业大学学报》2015年第4期433-439,共7页Journal of Shenyang Agricultural University

基　　金：国家科技部"十二五"国家科技支撑计划项目(2012BAK17B11);北京市教育委员会科技计划面上项目(KM201311417007);北京市教育委员会科技成果转化和产业化项目(PXM2013_014209_07-000082)

摘　　要：为研究不同提取方法和菌体浓度对类球红细菌辅酶Q10抗氧化活性的影响,测试了酶法辅助超声波法、超声波法、研磨法和纳米磨法对类球红细菌辅酶Q10的收率的影响。结果表明:同酶法辅助超声波法、超声波法和研磨法相比,纳米磨法提取类球红细菌辅酶Q10的收率最高。类球红细菌辅酶Q10具有一定的抗氧化能力,且与菌体浓度呈量效关系。纳米磨法、酶法辅助超声波法、超声波法和研磨法从类球红细菌提取的辅酶Q10均具有清除DPPH自由能力、抗脂质过氧化能力和还原能力,纳米磨法最好。固液比为1:10时,纳米磨法提取的辅酶Q10对DPPH自由基的清除率为(0.160±0.006)μmol Trolox·g-1,对脂质过氧化作用的抑制率为(56.4±2.4)%,还原力为0.563±0.020。The effects of both different extraction methods and biomass concentration on antioxidant activities of coenzyme Q10 from Rhodobacter sphaeroides were investigated in this paper. By comparison with grinding, ultrasonic and ultrasonic assisted with zymolysis, nano milling was the optimum extraction method for the yield of coenzyme Q10 from Rhodobacter sphaeroides 3757.The coenzyme Q10 extracted by the different methods from Rhodobacter sphaeroides 3757 exhibited significant antioxidant activities in vitro in a concentration-dependent manner. Compared with grinding, ultrasonic and ultrasonic assisted with zymolysis,the antioxidant activity of coenzyme Q10 extracted by nano milling from Rhodobacter sphaeroides 3757 was the best. As to nano milling, scavenging capacity against DPPH radical, lipid peroxidation inhibition activity and the reducing power were（0.1600 ±0.0056）μmol Trolox·g-1,（56.4±2.4）% and 0.563±0.020, respectively, when the material/liquid ratio was 1∶10.

关 键 词：类球红细菌 辅酶Q10 提取方法 纳米技术 抗氧化活性 

分 类 号：S432.4[农业科学—植物病理学]