凝胶色谱净化/气质联用法测定即食豆制品中二甲基黄  

Chromatography-mass spectrometry with gel permeation chromatography

作　　者：李桂生[1] 陈桂琴[1] 杨戈[1] 熊华亮[1] 

机构地区：[1]江西省分析测试研究所,南昌330029

出　　处：《分析试验室》2015年第12期1399-1402,共4页Chinese Journal of Analysis Laboratory

摘　　要：建立了凝胶渗透色谱结合气相色谱-质谱测定即食豆制品中二甲基黄的分析方法。样品采用正己烷提取，凝胶渗透色谱净化，经DB-5ms毛细管柱分离，选择离子监测模式（SIM）对目标化合物进行检测和确认。该方法在0．1～5μg／mL范围内线性关系良好，相关系数大于0．999。二甲基黄在O．5，1．0，1．5mg／kg3个添加水平的回收率为94．0％～101．0％，相对标准偏差为1．5％～2．2％。方法检出限为0．007mg／kg。方法适用于即食豆制品中二甲基黄的定性定量分析。A novel method was established for the rapid separation and determination of dimethyl yellow in Instant bean products. The target analytes were extracted with hexane and the extract was purified by gel permeation chromatography （GPC）. The analytes were separated on a DB-5ms column （30 m - 0.25 mm, 0. 25 μm） and analyzed in selected ion monitoring mode. An excellent linearity （ r 〉 0. 999） was observed in the concentration ranging from 0. 1 to 5 ug/mL for antioxidant. The recovery rates were ranged from 94. 0% to 101.0% at three levels of 0. 5, 1.0 and 1.5 mg/kg, with relative standard deviations （RSDs） of 1.5% 2. 2%. The limit of detection of dimethyl yellow was 0. 007mg,/kg. This method had good purifying effect, low time consuming, good accuracy, high sensitivity, and was suitable for the determination and confirmation of dimethyl yellow in Instant bean products.

关 键 词：凝胶渗透色谱 气相色谱-质谱联用 二甲基黄 

分 类 号：TS207.3[轻工技术与工程—食品科学]