量子点标记结合电化学免疫检测黄曲霉毒素B1  

Detection of Aflatoxin B1 Using Quantum Dots Mark and Electrochemical Immunoassay Technology

作　　者：朱浩嘉[1] 潘道东[1] 顾愿愿 李建龙[1] 赵紫微[1] 丁琳[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《中国食品学报》2015年第11期165-169,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家支撑计划(2012BAK08B01);宁波市创新团队项目(2012B82017);宁波市人事局人才基金项目(ZX2012000380)

摘　　要：将量子点标记技术与电化学检测技术相结合，基于竞争免疫分析，成功构建黄曲霉毒素B1（AFBl）检测新方法。以水相合成PbS量子点，通过TEM成像、XRD对其进行表征。随后以量子点标记AFB1单克隆抗体作为信号探针，基于酶标板表面AFB1人工抗原和样品中AFBI与量子点标记的AFBl单克隆抗体之间的竞争免疫结合，建立AFB1新型检测方法。在优化的条件下，方法线性范围0．1～30ng／mL，检测限0．03ng／mL。花生样品中加标回收试验结果表明，方法准确性良好，可用于实际样品的检测，为其它真菌毒素的检测提供参考。Combining Quantum dots labeled technique, electrochemical technology and competitive immunoassay technology, a new immune detection technology of aflatoxin B1（AFB1） was described. Primarily, the Pb S quantum dots was synthetized in aqueous phase and characterized by TEM, XRD. Then taked quantum dots labeled antibody as signal probes, the AFB1-BSA previously immobilised in the microtiter plate and AFB1 from the sample competitive conjugate signal probes, established a novel detection method for AFB1. Under the optimum conditions, the linear domain range was 0.1-30 ng/m L, the detection limit of 0.03 ng/m L. Results of recovery experiment in peanut sample showed that this method had a good accuracy and could be used for the detection of actual samples. The method could also be a valuable reference to other mycotoxins application.

关 键 词：黄曲霉毒素B1 电化学技术 量子点 竞争免疫分析 检测 

分 类 号：O657.1[理学—分析化学] TS207.3[理学—化学]