蛹虫草液体培养产纤溶酶条件优化研究  

Optimization of Submerged Culture Conditions for Production of Fibrinolytic Enzyme by Cordyceps militaris

作　　者：林群英[1,2] 封义 姜艳[3] 龙良鲲[3] 丁少军[3] 孙晓明[1,2] 

机构地区：[1]中华全国供销合作总社南京野生植物综合利用研究院,江苏南京210042 [2]江苏鸿丰果蔬食品有限公司,江苏宿迁223700 [3]南京林业大学化学工程学院,江苏南京210037

出　　处：《北方园艺》2015年第24期143-146,共4页Northern Horticulture

基　　金："十二五"国家科技支撑计划资助项目(2013BAD16B07)

摘　　要：利用蛋白纤维平板法对1株蛹虫草(Cordyceps militaris)菌株液体发酵产生纤溶酶的碳氮条件进行系统优化;通过单因素试验筛选最佳碳源和氮源,再根据单因素试验结果进行正交设计实验优化该菌株产纤溶酶的最佳配方。结果表明:产纤溶酶的最佳碳源为乳糖,纤溶酶活性达118.69U/mL,其次是不加碳源的空白对照,活性为109.12U/mL;蚕蛹粉是该菌株产纤溶酶的最佳氮源,活性可达118.69U/mL,其次是酵母浸膏,活性为29.10U/mL;纤溶酶产量与菌丝体生物量之间无必然相关性;产纤溶酶的最佳培养基为乳糖10g/L、蚕蛹粉10g/L、酵母浸膏5g/L,获得的纤溶酶活性高达142.26U/mL。Liquid culture conditions of carbon and nitrogen sources for production of fibrinolytic enzyme by aCodyceps militaris strain were optimized using fibrin plate method.The best carbon and nitrogen sources were sceened out by single factor method.Based on the outcomes of the single factor method,an othogonal test was designed to optimize the most suitable formula.The results showed that lactose was the best carbon source,with enzyme activity at 118.69U/mL,followed by the control without carbon source supplement,having activity at 109.12U/mL.The best nitrogen source was sildworm pupapowder,with the activity of 118.69U/mL and followed by yeast extract,giving enzyme activities of29.10U/mL.There was no certain relevance between enzyme activities and mycelial biomass.The optimal medium for fibrinolytic enzyme production consisted of lactose 10g/L,sildworm pupapowder 10g/L,yeast extract 5g/L.Highest activity of 142.26U/mL was obtained.

关 键 词：蛹虫草 纤溶酶 营养条件 深层发酵 

分 类 号：S567.35[农业科学—中草药栽培]