基于Box-Behnken设计的蛹虫草液体发酵培养基优化研究  

Study on Optimization of Mycelium Fermentation of Cordyceps militaris by Box-Behnken Design

作　　者：周广乙 王晓玲[1] 刘高强[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,林业生物技术湖南省重点实验室,长沙410004

出　　处：《食品工业》2015年第12期117-121,共5页The Food Industry

基　　金：国家948计划项目(2012-4-11);教育部“新世纪优秀人才支持计划”基金项目(NCET-13-1046);湖南省高校创新平台开放基金项目(14K112)

摘　　要：蛹虫草是具有重要开发价值的一种药用真菌。通过单因素试验确定了影响蛹虫草生物量的关键培养基因素,进一步基于Box-Behnken设计原理和响应面优化法对关键因素进行了优化。获得蛹虫草生物量(Y_1)的回归模型为Y_1=9.28+0.64A+0.29B+0.38C+0.21D+0.050AB+0.26AC-0.012AD+0.11BC+0.28BD+0.045CD-1.29A^2-0.45B^2-0.75C^2-0.72D^2。从中获得最佳培养基组成为:蔗糖31.48 g/L,蛋白胨5.84 g/L,酵母粉5.65 g/L,磷酸二氢钾1.61 g/L。经试验重复验证,蛹虫草菌丝体可达9.53 g/L,实测值与预测值十分接近,表明基于Box-Behnken设计原理获得的蛹虫草菌丝体发酵预测模型具有实践应用价值。Cordyceps militaris is a key medicinal fungus with great medicinal value. The liquid culture of C. militaris mycelium was optimized by means of Box-Behnken design and analyzed by response surface methodology. The results showed that the mathematical model for biomass production was Y_1=9.28＋0.64A＋0.29B＋0.38C＋0.21D＋0.050AB＋0.26AC-0.012AD＋ 0.11BC＋0.28BD＋0.045CD-1.29A^2-0.45B^2-0.75C^2-0.72D^2. Based on the model, the optimum medium for biomass production was that sucrose 31.48 g/L, peptone 5.84 g/L, yeast powder 5.65 g/L, and KH_2PO 1.61 g/L. The tested result of biomass yield was 9.53 g/L, which was very near to the predicted value. Suggesting the mathematical model for biomass production of C. militaris was useful in application.

关 键 词：蛹虫草 BOX-BEHNKEN设计 响应面法 液体发酵 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]