香茅精油的超临界CO_2萃取及其抗氧化活性的研究  

Study on citronella essential oil extraction with supercritical carbon dioxide and its antioxidant activity

作　　者：赵建芬[1] 李妍[1] 张素斌[1] 李绵盛 黎晓红[1] 

机构地区：[1]肇庆学院化学化工学院,肇庆526061

出　　处：《中国食品添加剂》2015年第12期98-103,共6页China Food Additives

基　　金：广东省高等学校优秀青年教师培养计划资助项目。项目名称:肇庆特色食品加工和资源利用(Yq2013164)

摘　　要：采用超临界CO_2萃取法提取香茅草中的精油,通过简单比较法优化萃取条件,并以香茅精油对DPPH自由基(DPPH·)、超氧阴离子自由基(O_2-·)和羟基自由基(OH·)的清除能力来研究其抗氧化活性。结果:在萃取压力为15MPa、萃取温度为45℃、萃取时间为90min的超临界CO_2萃取条件下香茅精油的得率最高,可达_2.13%。香茅精油在一定的浓度范围内其浓度与DPPH·、O_2-·和OH·清除率呈良好的线性关系,量效关系明显,对DPPH·、O_2-·和OH·的半抑制浓度(IC50)分别为3._237mg/m L、0._27_24mg/m L和0.6615mg/m L。表明:香茅精油具有良好的抗氧化活性,是一种开发潜力较高的天然抗氧化剂。The essential oil was extracted from Cymbopogoncitratus（DC.）Stapfby supercritical carbon dioxide,and extraction condition was optimized bysimple comparison experiments. The antioxidant activty of citronella essential oil was studied by scavenging free radical of DPPH·,O2-· and OH·.The results showed that the optimal extraction condition was extraction at 15 MPa,45℃ for 90 min,and the yield of essential oil was up to 2.13% under this condition. Within a certain range,linear relationship between free radical scavenging rate and the concentration of essential oil was good,and dose-effect relationship was obvious. The IC50 values of essential oil with free radicals of DPPH·,O2-· and OH· were 3.237mg/m L,0.2724mg/m L and 0.6615mg/m L,respectively. Conclusion： Citronella essential oil has a good antioxidant activty,it could be used as a natural antioxidant.

关 键 词：香茅精油 超临界CO2萃取 抗氧化 自由基 

分 类 号：TS202.3[轻工技术与工程—食品科学]