响应面法优化产油圆红冬孢酵母发酵培养基的研究  

Study of Culture Medium Optimization of the Oil Producing Rhodosporidium toruloides by Response Surface Methodology

作　　者：沙如意[1,2] 毛建卫[1,2] 蔡成岗[1,2] 刘士旺[1,2] 平丽娟[1,2] 

机构地区：[1]浙江科技学院生物与化学工程学院/轻工学院,浙江杭州310023 [2]浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023

出　　处：《食品与发酵科技》2015年第6期11-15,共5页Food and Fermentation Science & Technology

基　　金：浙江省教育厅科研项目(Y201327551);浙江科技学院科研启动基金项目(F501103C01);江苏省科技厅苏北科技专项资金(富民强县)项目(BN2014010)

摘　　要：采用部分因子设计筛选出影响产油圆红冬孢酵母生物量积累的主要因素为葡萄糖、酵母粉和硫酸铵。在此基础上利用响应面实验设计对主要影响因素进行分析,并预测出圆红冬孢酵母生物量产量最优的培养基组成。最后确定的最佳发酵培养基组成为(g/L):葡萄糖71.1,酵母粉2.19,硫酸铵2.83,磷酸二氢钾1,硫酸镁1,此时圆红冬孢酵母生物量为6.67g/L,与预测值6.62g/L非常接近,证实了该模型是合理的。The main factors influencing lipid-producing yeast Rhodosporidium toruloides biomass production were glucose, yeast extract and（NH4）2SO4by fractional factorial designs. Then the culture medium were optimized by response surface methodology design by determination of OD and biomass of the yeast. The optimal parameters were of（g/L）： glucose 71.1, yeast extract 2.19, ammonium sulfate 2.83, potassium dihydrogen phosphate 1 and magnesium sulfate 1. The biomass after cultivated in the optimal medium reached 6.67g/L, which had a small error with the predictive value of 6.62g/L, the model was proved to be reasonable.

关 键 词：优化 部分因子设计 圆红冬孢酵母 响应面设计 

分 类 号：TQ926.4[轻工技术与工程—发酵工程]