In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells  

作　　者：Yan Wang Ning Wang Biao Cai Guang-yun Wang Jing Li Xing-xing Piao 

机构地区：[1]Key Laboratory of Xin’an Medicine,Ministry of Education [2]Institute for Pharmacodynamics and Safety Evaluation of Chinese Medicine,Anhui Academy of Chinese [3]College of Pharmacy,Anhui University of Chinese Medicine

出　　处：《Neural Regeneration Research》2015年第12期2011-2017,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81374005,30973979;grant from the National Science and Technology Support Program during the Twelfth"Five-Year"Plan Period of China,No.2012BAI26B03

摘　　要：Drugs for the treatment and prevention of nervous system diseases must permeate the bloodbrain barrier to take effect.In vitro models of the blood-brain barrier are therefore important in the investigation of drug permeation mechanisms.However,to date,no unified method has been described for establishing a blood-brain barrier model.Here,we modified an in vitro model of the blood-brain barrier by seeding brain microvascular endothelial cells and astrocytes from newborn rats on a polyester Transwell cell culture membrane with 0.4-μm pores,and conducted transepithelial electrical resistance measurements,leakage tests and assays for specific bloodbrain barrier enzymes.We show that the permeability of our model is as low as that of the bloodbrain barrier in vivo.Our model will be a valuable tool in the study of the mechanisms of action of neuroprotective drugs.Drugs for the treatment and prevention of nervous system diseases must permeate the bloodbrain barrier to take effect.In vitro models of the blood-brain barrier are therefore important in the investigation of drug permeation mechanisms.However,to date,no unified method has been described for establishing a blood-brain barrier model.Here,we modified an in vitro model of the blood-brain barrier by seeding brain microvascular endothelial cells and astrocytes from newborn rats on a polyester Transwell cell culture membrane with 0.4-μm pores,and conducted transepithelial electrical resistance measurements,leakage tests and assays for specific bloodbrain barrier enzymes.We show that the permeability of our model is as low as that of the bloodbrain barrier in vivo.Our model will be a valuable tool in the study of the mechanisms of action of neuroprotective drugs.

关 键 词：nerve regeneration blood-brain barrier ASTROCYTES brain microvascular endothelial cells permeability CO-CULTURE Transwell chamber neural regeneration 

分 类 号：R743[医药卫生—神经病学与精神病学]