酶标抗原直接竞争ELISA检测食品中氟喹诺酮类药物多残留  

Direct Competitive ELISA with HRP-Labeled Antigen for Multiresidue Determination of Fluoroquinolones in Animal-Derived Food

作　　者：樊晓博[1] 谢兰心 

机构地区：[1]渭南职业技术学院渭南市农产品食品检验检测研究中心,陕西渭南714000

出　　处：《食品科学》2015年第24期265-269,共5页Food Science

基　　金：渭南职业技术学院青年科研基金项目(WZYQ201405)

摘　　要：固相包被恩诺沙星抗体,辣根过氧化物酶标记的抗原与标准品（或样品）中氟喹诺酮药物竞争结合抗体,建立了高效、高灵敏的氟喹诺酮药物直接竞争酶联免疫吸附分析检测方法.优化反应条件后,得到方法的IC50为2.04 μg/L,灵敏度为0.15 μg/L,线性范围0.3～15 μg/L;方法可以检测12种氟喹诺酮药物,在生乳、鸡肉、鱼肉和虾肉4种样品中12种药物的回收率为70％～121.5％.In this study, a rapid and high sensitive direct competitive enzyme-linked immunoassay （dc-ELISA） method based on antigen labeled by horseradish peroxidase （HRP） was established and successfully applied to detect fluoroquinolones （FQs） in animal-derived food. In the direct competitive assay, monoclonal antibody was bound to the surface of a microtiter plate, and the standards （or the sample） competed with antigen for the antibody binding sites. Under optimized assay conditions, the IC50 of dc-ELISA was 2.04 μg/L, the limit of detection was 0.15 μg/L, and the linear range was 0.3-15 μg/L. This method could detect 12 kinds of FQs with recoveries from milk, chicken, fish and shrimp in the range from 70% to 121.5%.

关 键 词：氟喹诺酮 酶标抗原 多残留检测 直接竞争酶联免疫吸附分析 

分 类 号：TS207.3[轻工技术与工程—食品科学]