利用茭白鞘叶水解木糖固定化米根霉发酵生产L-乳酸的研究  

L-lactic acid production with xylose derived from sheating leaf of Zizania latifolia by immobilized Rhizopus oryzae

作　　者：谢利波[1] 黄莹[1] 李鑫[1] 

机构地区：[1]台州学院生物质资源研究所,台州318000

出　　处：《食品科技》2015年第12期19-23,共5页Food Science and Technology

基　　金：新苗人才计划项目(2014R428030);台州学院青年基金项目(2014QN011);台州市课题(111KY18)

摘　　要：以茭白鞘叶为原料,改进了半纤维素水解以及木糖提取工艺,并在此基础上,提出了利用茭白鞘叶水解木糖固定化米根霉发酵生产L-乳酸的工艺,采用智能可视化软件(IVOS)优化发酵工艺条件。结果表明:当接种孢子量为0.013×10~6 cfu/m L、固定化载体的面积为15.2 cm^2、装液量为64 m L、水解木糖浓度为107 g/L时,固定化乳酸的生产强度达到了0.654 g/L·h,与游离米根霉相比(0.269 g/L·h),提高了1.43倍。This study investigated a novel strategy for L-lactic acid production by using immobilized Rhizopus oryzae on net with hemicelluloses hydrolysis of sheating leaf of Zizania latifolia,which obtained through the modified method of hemicelluloses hydrolysis and xylose extraction.Visualization method（IVOS） was used to optimize the process for L-lactic acid production.Results showed that when the inoculation spores concentration,net area,fermentation volume and hemicellulosic xylose concentration were 0.013×10~6 spores/m L,15.2 cm^2,64 m L and 107 g/L,respectively,the L-lactic acid productivity reached the highest（0.654 g/L·h）,which was higher 1.43 fold than free-cell fermentation（0.269 g/L·h）.

关 键 词：茭白鞘叶 米根霉 乳酸 固定化 水解木糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]