冠突散囊菌液体发酵工艺的研究  

Study on liquid fermentation technology of Eurotium cristatum

作　　者：李文娟[1] 吕嘉枥[1] 闫亚梅[1] 王霄鹏[1] 

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2016年第1期128-133,共6页Journal of Shaanxi University of Science & Technology

基　　金：陕西省教育厅专项科研计划项目(2010JK446);咸阳市科技计划项目(2013K06-09)

摘　　要：以陕西茯砖茶中优势菌群冠突散囊菌为实验菌株,采用液体深层发酵培养,研究了不同培养基成分、不同培养条件对菌丝球生长的影响.实验结果表明优化培养基配方为:土豆20%、蔗糖4%、硝酸铵0.3%、KH_2PO_40.15%、MgSO_4·7H_2O 0.03%;最适合菌丝球生长发育的理化因素为:培养温度28℃、摇瓶转速为120r/min、pH5.0、摇瓶装液量25%、发酵时间7d、种子培养时间为5d.Taking Eurotium cristatum,the superiority strains in Fuzhuan brick tea of Shaanxi,as test subjects,using liquid fermentation culture and L_9（3~4）orthogonal test to study the effects on mycelia by various media and conditions.The result showed that the optimal culture medium was：potato 20%,sucrose 4%,ammonium nitrate 0.3%,KH_2PO_4 0.15%,MgSO_4·7H_2O 0.03%.The optimal fermented condition was：culture temperature 28 ℃,rotation speed 120r/min,pH5.0,the amount of liquid 125mL（500mL canonicalflask）,incubation time for seed liquid 5days,and fermentation time 7days.

关 键 词：冠突散囊菌 液体发酵 菌丝体 

分 类 号：TS201.3[轻工技术与工程—食品科学]