酵母细胞壁β-D-葡聚糖定量测定方法的比较分析  

Comparative Analysis of Quantitative Assay Methods for β-D-Glucan from Yeast Cell Walls

作　　者：田晓丽[1,2] 杨萍[1] 姜文侠[1] 

机构地区：[1]天津市工业生物系统与过程工程重点实验室,中国科学院系统微生物工程重点实验室,中国科学院天津工业生物技术研究所,天津300308 [2]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品科学》2016年第2期99-103,共5页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2012AA021403)

摘　　要：通过对酵母胞壁不溶性β-D-葡聚糖的几种定量测定方法的比较分析,表明经酸水解法处理后得到的测定结果偏高,经酸-酶水解法处理后的测定结果更准确;对水解后产生的葡萄糖采用生物传感仪法定量,结果稳定,操作时间短,更适合在生产过程和质量控制中使用;待测样品中的α-葡聚糖对测定结果有明显的干扰,β-D-葡聚糖含量应为总葡聚糖含量与α-葡聚糖含量之差。This study was conducted to compare and analyze several quantitative assay methods for insoluble β-D-glucan from yeast cell walls. It was indicated that the sample pretreatment by acid hydrolysis provided higher results for the determination of β-D-glucan, while the results obtained by acid-enzymatic hydrolysis were more precise. Among different test methods for glucose obtained after hydrolysis, the biosensor method was found to be faster, more stable and suitable for quality control in the production process. Besides, α-glucan in the samples had a significant interference effect on the results of determination. Thus β-Dglucan should be calculated by subtraction from total glucose of the glucose obtained from α-glucan.

关 键 词：Β-D-葡聚糖 酵母细胞壁 水解方法 定量测定 生物传感仪法 

分 类 号：Q539[生物学—生物化学]