响应曲面法优化黑青稞花青素的提取工艺  

Optimization of Extraction Process of Anthocyanin from Nangqian Black Hulless barley by Response Surface Methodologyl

作　　者：陈建国[1] 梁寒峭[1] 李金霞[1] 程池[1] 郭积新 

机构地区：[1]中国食品发酵工业研究院,北京100015 [2]青海省玉树州囊谦县农牧科技水务局,囊谦815200

出　　处：《食品工业》2016年第1期80-83,共4页The Food Industry

摘　　要：以囊谦黑青稞原料,在单因素试验基础上,以乙醇体积分数、液料比、提取温度、提取时间和pH为响应因素,利用Box-Behnken中心组合试验和响应面分析法,研究了各自变量交互作用及其对花青素提取量的影响,模拟得到二次多项式回归方程的预测模型。最佳提取工艺条件为:乙醇体积分数59.01%、液料比21.87∶1(m L/g)、提取温度62.40℃、提取时间2.76 h、pH 1.82。在此条件下,花青素的提取实测结果 (103.48μg/g)与响应曲面拟合所得方程的预测值(105.93μg/g)符合良好。Based on the experiment of single factor,the mathematical regression model was established about the dependent variable（extraction yield of anthocyanin from Nangqian black Hulless barley） and independent variables（ethanol concentration,ratio of liquid to solid,extraction temperature,extraction time,pH） through Box-Behnken experimental design combined with response surface methodology.The optimum extraction condition was as follows：ethanol concentration of 59.01%,ratio of liquid to solid of 21.87：1（mL/g）,extraction temperature 62.40℃,extraction time 2.76 h,pH was 1.82.Under this condition,the practical yield of anthocyanin was 103.48 μg/g,which was consistent with the predicted value of 105.93 μg/g.

关 键 词：黑青稞 囊谦 花青素 响应曲面法 提取工艺 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]