高产γ-氨基丁酸乳酸菌菌株的诱变选育与鉴定  

Study on mutation breeding and identification of lactic acid bacteria for γ- aminobutyric acid production

作　　者：双全[1] 万月[1] 刘俐[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018

出　　处：《食品工业科技》2016年第3期153-155,159,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(31460443)

摘　　要：本文以从内蒙古传统发酵食品中分离的80株乳酸菌为研究对象,在GYP培养基中进行高产γ-氨基丁酸(GABA)菌株的筛选后,利用紫外线进行诱变处理,得到GABA突变菌株,并对其进行了菌种鉴定。结果表明,从80株供试乳酸菌中筛选出4株高产γ-氨基丁酸的菌株,再经紫外诱变后得到1株高产突变菌株US3-3。该菌株紫外诱变后,其γ-氨基丁酸含量为2.482 g/L,是诱变前提高1.9倍,并对其多次传代稳定性较好,经16S r DNA序列分析,鉴定为乳酸片球菌(Pediococcus acidilactici)。The growth ability of 80 lactic acid bacteria strains isolated from the traditional fermented food in Inner Mongolia were screened according to the γ- aminobuyric acid（ GABA） accumulation amount in the GYP medium.The high- yield GABA strains were obtained by ultraviolet mutagenesis,and then the strain was identified by16 S r DNA gene sequences.The results showed that 4 high- yield GABA strains were screened out from 80 strains of lactic acid bacteria and a high yield GABA producing strain US3- 3 was obtained by UV mutagenesis and its content of GABA was 2.482 g / L,which was 1.9 times as much as original strain.The strain US3-3 was identified as Pediococcus acidilactici.

关 键 词：Γ-氨基丁酸 乳酸菌 紫外诱变 鉴定 

分 类 号：TS252.5[轻工技术与工程—农产品加工及贮藏工程]