基于UPLC-MS-MS检测白酒中辛硫磷含量及安全评估  

Discussion on Security Evaluate About Phoxim Determining in Liquor Based on UPLC-MS-MS

作　　者：唐林[1] 王银辉[1,2] 汤有宏 刘国英[1,2] 李安军[1] 周庆伍[1] 

机构地区：[1]安徽古井贡酒股份有限公司,安徽亳州236820 [2]安徽省固态发酵工程技术研究中心,安徽亳州236820

出　　处：《酿酒》2016年第1期68-70,共3页Liquor Making

摘　　要：建立了UPLC-MS-MS测定白酒中辛硫磷含量的测定方法。试样以ACQUITY UPLC BEH C18色谱柱为分离柱,以甲醇和5m M乙酸铵水溶液作为流动相进行梯度洗脱,多反应（MRM）模式检测。结果表明,辛硫磷在10~200ng/m L范围内线性关系良好,线性相关系数（R2）为0.998。3个添加水平的回收率范围为98.50%~101.80%,相对标准偏差均控制在5%以内。方法简单快捷、准确可靠,可用于白酒产品质量安全控制和风险评估。A sensitive method based on UPLC- MS- MS has been developed for the determination of phoxim in liquor. The sample was loaded onto a ACQUITY UPLC BEH C18 column（2.1 mm×100 mm,1.7μm）by gradient elution using 5mmol/L ammonium acetate solution/methanol as mobile phase in ultra performance liquid chromatography（UPLC）, followed by multiple reaction monitoring mode（MRM）. The linear range of standard curve for phoxim is from 10 to 200ng/m L, and the linear relationship was 99.8%. The spiked recoveries are within 98.5%~101.8% in three level of standard addition among linear range of standard curve. Then, the relative standard deviation（RSD） are controlled below 5%. The method is simple, quick, accurate and reliable, and can be used for liquor product quality safety control and risk assessment.

关 键 词：UPLC-MS-MS 辛硫磷 白酒 安全评估 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS207.3[轻工技术与工程—食品科学与工程]