Hepatocyte transplantation program:Lessons learned andfuture strategies  

作　　者：Eugenia Pareja Ibars Miriam Cortes Laia Tolosa Maria JoséGómez-Lechón Slivia López JoséVicente Castell JoséMir 

机构地区：[1]Unidad de Terapia Celular Hepatica,Instituto de Investigación Sanitaria La Fe,Hospital Universitario y Politécnico La Fe de Valencia,46026 Valencia,Spain [2]Unidad de Cirugía Hepato-Bilio-Pancreática y Trasplante Hepático,Hospital Universitario y Politécnico La Fe de Valencia,46026 Valencia,Spain [3]Liver transplantation,Institute of Liver Studies,King’s College Hospital,Institute of Liver Studies,SE5 9RS London,United Kingdom [4]Unidad de Hepatologıa Experimental,Instituto de Investigación Sanitaria La Fe,Hospital Universitario y Politécnico La Fe de Valencia,46026 Valencia,Spain [5]CIBERehd,FIS,08036 Barcelona,Spain

出　　处：《World Journal of Gastroenterology》2016年第2期874-886,共13页世界胃肠病学杂志（英文版）

摘　　要：This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital La Fe in Valencia. New sources of liver tissue for hepatocyte isolation have been explored. The hepatocyte isolation and cryopreservation procedures have been optimized and quality criteria for assessment of functionality of hepatocyte preparations and suitability for HT have been established. The results indicate that:(1) Only highly viable and functional hepatocytes allow to recover those functions lacking in the native liver;(2) Organs with steatosis(≥ 40%) and from elderly donors are declined since low hepatocyte yields, viability and cell survival after cryopreservation, are obtained;(3) Neonatal hepatocytes are cryopreserved without significant loss of viability or function representing high-quality cells to improve human HT;(4) Cryopreservation has the advantage of providing hepatocytes constantly available and of allowing the quality evaluation and suitability for transplantation; and(5) Our results from 5 adults with acute liver failure and 4 from children with inborn metabolic diseases, indicate that HT could be a veryuseful and safe cell therapy, as long as viable and metabolically functional human hepatocytes are used.This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital La Fe in Valencia. New sources of liver tissue for hepatocyte isolation have been explored. The hepatocyte isolation and cryopreservation procedures have been optimized and quality criteria for assessment of functionality of hepatocyte preparations and suitability for HT have been established. The results indicate that:(1) Only highly viable and functional hepatocytes allow to recover those functions lacking in the native liver;(2) Organs with steatosis(≥ 40%) and from elderly donors are declined since low hepatocyte yields, viability and cell survival after cryopreservation, are obtained;(3) Neonatal hepatocytes are cryopreserved without significant loss of viability or function representing high-quality cells to improve human HT;(4) Cryopreservation has the advantage of providing hepatocytes constantly available and of allowing the quality evaluation and suitability for transplantation; and(5) Our results from 5 adults with acute liver failure and 4 from children with inborn metabolic diseases, indicate that HT could be a veryuseful and safe cell therapy, as long as viable and metabolically functional human hepatocytes are used.

关 键 词：HEPATOCYTE TRANSPLANTATION Hepatocyteisolation Cell therapy INBORN errors of metabolism NEONATAL HEPATOCYTES CRYOPRESERVATION 

分 类 号：R575[医药卫生—消化系统]