响应面法优化带鱼蛋白多肽螯合亚铁制备工艺  

Optimization of preparation conditions of hairtail protein polypeptide-Iron(Ⅱ) chelate by response surface methodology

作　　者：黄赛博 林慧敏[1] 邓尚贵[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316022

出　　处：《食品工业科技》2016年第4期266-270,277,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31301597);国家海洋公益性行业科研专项(201305013)

摘　　要：以带鱼鱼糜为原料,研究带鱼蛋白多肽和亚铁离子的螯合工艺条件。分别以螯合反应p H、螯合温度、时间、Fe Cl2溶液与酶解液体积比、抗坏血酸添加量为主要研究对象,在单因素实验进行螯合率测定的基础上,通过Box-Behnken设计响应面优化实验。结果显示,螯合反应的最佳工艺参数为p H6.9,温度29℃,螯合时间30 min,Fe Cl_2溶液与酶解液体积比为1∶50,抗坏血酸添加量为酶解液质量的0.1%,该条件下得到的螯合率为80.46%。红外图谱研究表明,螯合前后结构发生改变,证明带鱼蛋白多肽和亚铁离子确实形成新的螯合物。The preparation conditions of hairtail protein polypeptides chelating iron（Ⅱ） were studied,with hairtail surimi as raw materials. The influences of p H,temperature,time,solution volume ratio of Fe Cl_2 to enzymatic hydrolysate and ascorbic acid content on the yield of peptide- iron（ Ⅱ） chelate were investigated. The optimization experiments of response surface were designed by Box-Behnken based on above one-factor experiments. The results showed that the optimal chelating conditions were p H6.9,29 ℃,30 min,Fe Cl_2-enzymatic hydrolysate volume ratio 1∶50（V/V）,and ascorbic acid content 0.1% of hydrolysate. Under these conditions,the yield of peptide-iron（Ⅱ） chelate was 80.46%. The analysis of infrared spectrum revealed that the ferrous ions were chelated with the amino groups and carboxyl groups,which suggested the formation of a new kind of peptide-iron（Ⅱ） chelate.

关 键 词：带鱼鱼糜 多肽亚铁螯合物 响应面 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程]