牡蛎肽抗氧化和对T淋巴细胞增殖作用的影响  

Effect of oyster oligopeptide antioxidant activity and T-lymphocyteproliferation in vitro

作　　者：魏颖[1,2] 陈亮[1,2] 孙玉坤[3] 金振涛[1,2] 王憬[1,2] 刘艳[1,2] 蔡木易[1,2] 

机构地区：[1]中国食品发酵工业研究院,北京100027 [2]北京市蛋白功能肽工程技术研究中心,北京100027 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科技》2016年第1期209-212,共4页Food Science and Technology

基　　金：国家十二五科技支撑项目(2012BAD33B04-02);国家高技术研究发展计划(863计划)项目(2013AA102205-02);科技北京百名领军人才培养工程项目(Z131110000513026);北京市科技计划项目(Z131100003113010)

摘　　要：目的:主要对牡蛎肽的基本理化指标进行了测定,并对其清除细胞内ROS含量的能力和T淋巴细胞增殖影响进行研究。方法:采用AAPH[2,2’-Azobis(2-methylpropionamidine)dihydrochloride]诱导的Hep G2细胞氧化应激模型评价牡蛎肽缓解细胞内ROS水平的影响,应用刀豆蛋白(Con A)诱导小鼠T-淋巴细胞增殖,选取WST-8法检测不同浓度牡蛎肽对小鼠脾细胞和T淋巴细胞增殖的影响。结果:牡蛎肽主要含有相对分子量低于1000 u的八肽以下的短肽,具有抑制细胞内ROS生成的能力,并对Con A诱导的T-淋巴细胞增殖有抑制作用。结论:牡蛎肽具有显著的抗氧化能力和抑制T淋巴细胞增殖作用。Objective： The basic physical and chemical indicators of oyster oligopeptide were measured, and its antioxidant activity and T-lymphocyte proliferation in vitro was study. Methods： The ROS level in cell was used to evaluate the antioxidant activity of oyster oligopeptide on AAPH-induced in Hep G2 cells model. The effect of sample on the T-lymphocyte proliferation was detected by WST-8. Results： The relative molecular weight of oyster oligopeptide was less than 1000 u. Oyster oligopeptidehad the higher ability to scavenge lipid peroxidation of free radicals and inhibited Con A-induced T-lymphocyte proliferation ef fect. Conclusion： Oyster oligopeptide has good antioxidant activity, and inhibited T-lymphocyte proliferation.

关 键 词：牡蛎肽 抗氧化 T淋巴细胞 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]