浓香型白酒窖池窖泥中原核微生物菌群的研究  

Prokaryotic Bacteria Communities in Pit Mud of Nongxiang Baijiu

作　　者：罗杰 张宿义[1,2,3] 敖宗华[2,3] 王松涛[2] 税梁扬 杨艳 李德林 张兵 

机构地区：[1]泸州龙泉窖酒业有限公司,四川泸州646606 [2]泸州老窖股份有限公司,四川泸州646000 [3]国家固态酿造工程技术研究中心,四川泸州646000 [4]泸州科源生物科技有限公司,四川泸州646606

出　　处：《酿酒科技》2016年第2期55-60,共6页Liquor-Making Science & Technology

摘　　要：提取浓香型窖池窖壁泥和窖底泥样品总DNA,构建窖泥细菌16S rRNA文库,经过16S r RNA扩增片段的限制性酶切分析(ARDRA)、DNA序列测定和系统发育关系分析,对比研究了窖壁泥和窖底泥细菌群落组成。数据分析结果表明,窖池窖壁泥和窖底泥中细菌种类及数量的差异性较大。窖壁泥中优势菌属为Clostridiumsp、Caloramatorsp和Desulfotoma-culum carboxydivorans,所占比例分别为17%、8%和6%。窖底泥优势菌属为Clostridium acidurici、Ruminococcus bromii、Proteiniphilumsp和Parabacteroides merdae,所占比例分别为18%、8%、7%和6%。初步揭示了窖池窖壁泥和窖底泥中细菌系统发育多样性及相互之间存在的差异。Total DNA were extracted from pit wall mud and pit bottom mud for Nongxiang Baijiu production for the construction of clone libraries of 16 S r RNA.The bacteria composition and diversity in pit mud between pit wall and pit bottom were contrasted by amplified 16 S rRNA restriction analysis（ARDRA）, DNA sequencing and phylogenetic analysis.The results showed that there was significant difference in the varieties and the quantity of bacteria from pit wall mud and pit bottom mud, the dominant bacteria species in pit wall mud included Clostridiumsp, Caloramatorsp and Desulfotomaculum carboxydivorans（17 %, 8 % and 6 % of total bacteria quantity）, the dominant bacteria species in pit bottom mud included Clostridium acidurici, Ruminococcus bromii, Proteiniphilumsp and Parabacteroides merdae（18 %, 8 %, 7 % and 6 %of total bacteria quantity）.This research preliminarily revealed the difference in bacteria diversity between pit wall mud and pit bottom mud.

关 键 词：窖泥 16S RRNA序列 克隆文库 白酒 

分 类 号：TS261.1[轻工技术与工程—发酵工程]