4个葡萄品种葡萄籽冷榨油的性质与体外抗氧化活性  

Characterization and in Vitro Antioxidant Activity of Cold-Pressed Seed Oils from Four Different Grape Varieties

作　　者：郑亚蕾[1] 刘叶[1] 隋银强[1] 王华[1,2,3] 李华[1,2,3] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100 [3]合阳葡萄试验示范站,陕西渭南715300

出　　处：《食品科学》2016年第3期27-32,共6页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD31B00);西北农林科技大学2013年试验示范站(基地)科技创新与成果转化项目(XNY2013-64);科技部火炬计划(杨凌)葡萄酒产业技术升级公共平台项目(2011GH551976)

摘　　要：目的：评价4种葡萄籽冷榨油脂肪酸组成、总酚含量及体外抗氧化活性。方法：收集单品种葡萄籽榨油,气相色谱法测定脂肪酸组成,Folin-酚法测定总酚含量,用1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picryhydrazyl,DPPH）法、2,2’-联氮双-（3-乙基苯并噻唑林-6-磺酸）二胺盐（2,2’-azinobis（3-ethylbenzothi azoline-6-sulfonic acid）ammonium salt,ABTS）法、Fenton反应和总抗氧化能力法分析其体外抗氧化能力。结果：4个品种葡萄籽冷榨油均富含亚油酸（72.77%~77.36%）,不饱和脂肪酸含量为88.12%~91.06%;总酚含量为60.77~100.53μg GAE/g,爱格丽葡萄籽冷榨油总酚含量最高,其次是北冰红、媚丽、赤霞珠葡萄籽冷榨油;4个品种的葡萄籽冷榨油都有良好的清除DPPH自由基和ABTS＋·的能力,且随着葡萄籽冷榨油浓度的增大,清除能力增强,清除DPPH自由基的IC50变化范围为10.19~13.82 mg/m L,清除ABTS＋·的IC50变化范围为11.62~19.25 mg/m L;清除羟自由基（·OH）的能力范围为13.18~27.08 U/m L;总抗氧化能力范围为1.08~3.68 U/m L。4个品种的葡萄籽冷榨油的体外抗氧化能力强弱顺序为爱格丽〉北冰红〉媚丽〉赤霞珠。Objective： To evaluate the fatty acid composition and total polyphenol contents of cold-pressed seed oils from four different grape varieties and assay their in vitro antioxidant activity. Methods： The fatty acid composition was measured by gas chromatography with fl ame ionization detection（GC-FID）. The content of polyphenols was determined using the Folin-Ciocalteau method and the in vitro antioxidant activity was tested by 1,1-diphenyl-2-picryhydrazyl（DPPH） radical scavenging, 2,2＇-azinobis-3-ethylbenzthi azoline-6-sulfonic acid（ABTS） radical scavenging and Fenton reaction methods. Results： The most abundant fatty acid in all cold-pressed grape seed oils was linoleic acid（72.77%–77.36%） and the content of unsaturated fatty acids ranged from 88.12% to 91.06%. Total polyphenols ranged from 60.77 to 100.53 μg GAE/g and the order of total polyphenol contents in grape seed oils from different grape varieties was Ecolly Beibinghong Meili Carbernet Sauvignon. All clod-pressed grape seed oils had strong scavenging activity against DPPH, ABTS and hydroxyl free radicals in a dose-dependent manner, with IC50 values ranging from 10.19 to 13.82 mg/m L, from 11.62 to 19.25 mg/m L, and from 13.18 to 27.08 U/m L, respectively. The IC50 value of total antioxidant capacity ranged from 1.08 to 3.68 U/m L. The order of in vitro antioxidant activity of cold-pressed grape seed oils was Ecolly 〉Beibinghong 〉Meili 〉Carbernet Sauvignon.

关 键 词：葡萄籽冷榨油 多酚 体外抗氧化活性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]