暗纹东方鲀肌肉组织蛋白酶B提取工艺优化  

Optimization of Extraction Conditions for Cathepsin B from Takifugu obscures Muscle

作　　者：李琳[1] 王正全[1] 张晶晶[1] 苗晓丹[1] 马垒[1] 王锡昌[1] 刘源[1] 

机构地区：[1]上海海洋大学食品学院,上海水产品加工及贮藏工程技术研究中心,上海201306

出　　处：《食品科学》2016年第3期91-96,共6页Food Science

基　　金：国家自然科学基金面上项目(31371790;31271900);“十二五”国家科技支撑计划项目(2012BAD28B01);上海市科委工程中心建设项目(11DZ2280300);上海市教委重点学科建设项目(J50704)

摘　　要：为获得暗纹东方鲀组织蛋白酶B的最佳提取工艺条件,在单因素试验的基础上,通过Plackett-Burman设计对影响组织蛋白酶B活力的因素进行筛选,结果表明:料液比、磷酸盐缓冲液p H值和硫酸铵饱和度这3个因素显著影响酶活力,然后用响应面分析法确定了主要影响因素的最佳提取条件为:料液比1∶2.1、磷酸盐缓冲液p H 7.0、硫酸铵饱和度99.2%,在此条件下组织蛋白酶B活力的预测值为32.65 U/g,实测值为32.52 U/g,相对误差为0.39%。说明建立的模型切实可行,为进一步研究组织蛋白酶B的提取工艺提供理论依据。In order to obtain optimal conditions for the extraction of cathepsin B from Takifugu obscures, on the basis of results of single-factor experiments, some extraction parameters affecting cathepsin B activity were firstly evaluated by Plackett-Burman design, and then the main variables were optimized by response surface analysis. The results showed that the optimal extraction conditions were as follows： material/liquid ratio 1：2.1, phosphate buffer p H 7.0, and ammonium sulfate saturation 99.2%, and these three factors had signifi cant infl uence on the enzyme activity. Under these conditions, the predicted value of cathepsin B activity was 32.65 U/g and its experimental value was 32.52 U/g, suggesting that the established model is feasible and can provide a theoretical basis for further studies on the extraction process of cathepsin B.

关 键 词：暗纹东方鲀 组织蛋白酶B PLACKETT-BURMAN设计 响应面分析法 

分 类 号：TS201[轻工技术与工程—食品科学]