酸马奶提取植物乳杆菌DSM20174细菌素的理化特性研究  

Study on Physicochemical Properties of Lactobacillus plantarum DSM20174 Bacteriocin from Koumiss

作　　者：姜晶[1] 敖日格乐[1] 王纯洁[2] 张燕[1] 赵称赫 吴宏军 郝剑刚 

机构地区：[1]内蒙古农业大学动物科技学院,呼和浩特010018 [2]内蒙古农业大学兽医学院,呼和浩特010018 [3]呼伦贝尔市海拉尔谢尔塔拉牧场,海拉尔021012 [4]内蒙古贺斯格绿色产业进出口有限公司,锡林郭勒023126

出　　处：《中国畜牧兽医》2016年第2期444-449,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31060318;31260570);国家科技支撑项目(2012BAD12B09-02)

摘　　要：为研究从酸马奶中提取的植物乳杆菌DSM20174细菌素的理化特性,本试验提纯植物乳杆菌DSM20174细菌素,经过硫酸铵沉淀、透析后得到细菌素粗提液,再利用SephadexG-100凝胶柱层析进行纯化后,细菌素的比活力明显增加,达到154.70AU/mL,得率为43.5%。采用牛津杯法测定纯化的植物乳杆菌DSM20174细菌素在3种不同方式处理下对牛源野生致病性大肠杆菌O78抑菌效果的影响。结果显示：1植物乳杆菌DSM20174细菌素在5组温度处理下,随着温度的升高抑菌作用降低,各组抑菌作用差异显著（P〈0.05）。即使121℃处理30min后,抑菌直径仍达14.90mm。2植物乳杆菌DSM20174细菌素在pH 2.0-12.0由低到高的11组酸碱处理下,pH越大抑菌直径越小,且除了pH 9.0和pH 10.0之间差异不显著（P〉0.05）外,其余各组抑菌作用均差异显著（P〈0.05）。3植物乳杆菌DSM20174细菌素经胰蛋白酶、胃蛋白酶、木瓜蛋白酶3种酶处理后抑菌直径变化明显,处理前后抑菌作用均差异显著（P〈0.05）。结果表明植物乳杆菌DSM20174细菌素是较好的抑菌活性物质,但不是热稳定性物质;具有较广泛的pH适用范围,且pH越小时其抑菌活性越强,在最适生长pH时抑菌活性有所增强;不是蛋白酶稳定性物质。To study the physicochemical properties extracted from koumiss of Lactobacillus plantarum DSM20174 bacteriocin,we purified the Lactobacillus plantarum DSM20174 bacteriocins and got the bacteriocin crude extractions after ammonium sulfate precipitation and dialysis.After purification with Sephadex G-100 gel column chromatography,bacteriocin specific activity apparently increased,reaching 154.70 AU/mL,yield was 43.5%.We detected the effects of purified Lactobacillus plantarum DSM20174 bacteriocin on bacteriostatic action of wild bovine pathogenic E.coli O78 by Oxford cup method under 3different treatments.The results showed that：1In five groups of temperature treatments,the bacteriostatic action decreased with the increase of temper-ature,bacteriostatic actions of all groups were significantly different（P〈0.05）.Even after treatment at 121 ℃ 30 min,antimicrobial diameter was 14.90 mm.2 After Lactobacillus plantarum DSM20174 bacteriocin were treated with pH 2.0to pH 12.0,the higher of pH,the smaller of antibacterial diameter.The inhibitory effect of groups were significantly different（P〈0.05）except pH 9.0and pH 10.0.3Lactobacillus plantarum DSM20174 bacteriocins were treated by trypsin,pepsin and papain,antibacterial diameter apparently changed,bacteriostatic action was significantly different（P〈0.05）before and after treatment.The results suggested that Lactobacillus plantarum DSM20174 bacteriocins were better antibacterial substances,but not thermal stability substances.It had a relatively wide pH range,and the smaller of pH,the stronger of its antibacterial activity,the antibacterial activity had been enhanced at optimum growth pH.It was not protease stability substances.

关 键 词：植物乳酸杆菌DSM20174细菌素 牛源野生致病性大肠杆菌O78 抑菌活性 

分 类 号：S852.4[农业科学—基础兽医学]