正交试验优化龙眼核中原花青素的提取工艺  

Optimization of Extracting Proanthocyanidins from Longan Stone by Orthogonal Test

作　　者：黄海潮[1] 刘经亮[1] 郑公铭[1] 朱庆玲[1] 周捷[2] 

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]中山大学肿瘤防治中心药学部,广东广州510060

出　　处：《黑龙江医药》2015年第6期1228-1231,共4页Heilongjiang Medicine journal

基　　金：广东省科技计划项目(编号:2010B020312006);广东省医学科学技术研究基金项目(B2013071)

摘　　要：目的：研究从经处理后的龙眼核中提取原花青素的最佳工艺条件。方法：以提取时间、温度、提取溶剂的体积分数以及提取的料液比进行单因素试验,并以此为依据进行L_9（3~4）正交试验,优化得出最佳的提取条件。结果：研究表明各因素中对龙眼核原花青素提取影响的大小次序为：提取时间〉料液比〉乙醇体积分数〉提取温度,其最佳提取工艺为：50%乙醇,料液比1：10、提取时间2h以及提取温度60℃。结论：正交设计优化得到的龙眼核原花青素提取工艺方法具有更佳的提取效率。Objective： To study the most effective condition of extracting Proanthocyanidins from Longan stone.Methods： Single factor experiments of extracting procyanidins from Longan stone was carried out including extracting period, Temperature, volume frac- tion of extracted solvent and solid/liquid ratio .To find the most effective condition of extracting proanthocyanidins by Orthogonal array design L（34）. Results： the most optimal extraction conditions were found to be using 50% ethanol with a solid/liquid ratio of 1：10 and extraction at 60 ~C for 2h, and the effect of four important variables on the extracting rate of proanthocyanidins followed decreasing or- der： extracting duration 〉 solid/liquid ratio 〉ethanol concentration 〉temperature. Conclusion：Extraction condition of Proanthocyani- dins from longan stone by orthogonal test had better extraction efficiency

关 键 词：原花青素 龙眼核 正交试验 工艺条件 乙醇体积分数 体积分 提取效率 生物类黄酮 正交法 提取率 

分 类 号：R284.2[医药卫生—中药学]