直接进样气相色谱-质谱法快速测定酒中氨基甲酸乙酯  

Rapid determination of ethyl carbamate in liquor by direct injection coupling with GC-MS

作　　者：周琛[1] 陈汉哲[1] 苏婷[1] 王文洋[1] 周韩玲[2] 廖勤俭 郭艳[2] 郑波[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]五粮液集团有限公司,四川宜宾644007

出　　处：《现代预防医学》2016年第4期715-717,721,共4页Modern Preventive Medicine

摘　　要：目的建立直接进样气相色谱-质谱检测法（GC-MS）测定白酒中氨基甲酸乙酯方法。方法采用1.0μl直接进样,DB-WAX毛细管柱程序升温分离,SIM模式检测,定量离子（m/z）62,内标标准曲线法定量。结果本法在0.02～15.0μg/ml的范围内线性关系良好（r=0.999）,日内和日间精密度分别为0.480%和2.60%,方法的加标回收率在97.0%～107%之间,方法的检出限和定量限分别5.00μg/L和20.0μg/L。结论所建立的方法快速、准确、灵敏,能满足白酒中氨基甲酸乙酯的常规分析。Objective The study aimed to establish a gas chromatography-mass spectrometry （GC-MS） method for detection of ethyl carbamate in liquor. Methods 1.0 μl of samples were directly injected into a GC-MS and separated on a DB-WAX capillary column using a temperature gradient program. The separated compounds were detected under the SIM mode and ion having a m/z of 62 was quantitated based on an internal standard curve. Results Good linearity with a linear correlation coefficient r of 0.999 was exhibited within 0.02-15.0 μg/ml. The intra- and inter-day precisions were 0.480% and 2.60%, respectively. Recovery rate of the samples ranged between 97.0% and 107%. Detection limit and quantitation limit of the method were 5.00 μg/L and 20.0 μg/L, respectively. Conclusion The established method is fast, accurate, sensitive, and meets the requirements for routine analysis of ethyl carbamate in liquor.

关 键 词：气相色谱质谱法 氨基甲酸乙酯 白酒 

分 类 号：R155.6[医药卫生—营养与食品卫生学]