咖啡对血小板糖蛋白ⅡbⅢa、CD40L表达的急性影响  

ACUTE EFFECT OF COFFEE ON EXPRESSION OF PLATELET GLYCOPROTEINS ⅡbⅢa,CD40L

作　　者：刘磊[1] 潘娟娟[1] 凌怡[1] 潘力健[1] 龚辉[1] 

机构地区：[1]复旦大学附属金山医院心内科,上海201508

出　　处：《营养学报》2016年第1期48-51,共4页Acta Nutrimenta Sinica

基　　金：上海复旦大学附属金山医院课研项目(No.201307)

摘　　要：目的研究咖啡对血小板功能的影响,阐明其中可能的机制。方法 12名健康男性被随机分配到三组之一,进行为期3w的交叉试验,分别饮用相同咖啡因含量(180 mg)的咖啡、咖啡因片剂或安慰剂。分别在即刻0、2、4h采血,对血小板进行全血流式细胞仪分析,测定血小板活化状态糖蛋白ⅡbⅢa(glycoprotein ⅡbⅢa,GPⅡbⅢa)、CD40L表达。另在体外,测定不同浓度咖啡因对血小板GPⅡbⅢa影响。结果与对照组比较,饮用咖啡抑制二磷酸腺苷(ADP)诱导的GPⅡbⅢa表达(2h和4h,P<0.05)和CD40L表达(2h和4h,P<0.05)。咖啡也抑制花生四烯酸诱导GPⅡbⅢa表达(2h和4h,P<0.05)和CD40L表达(2h,P<0.05)。安全剂量咖啡因的摄入不影响血小板活化后GPⅡbⅢa和CD40L表达。结论咖啡在体内可以通过减少血小板GPⅡbⅢa、CD40L表达,进而调节血小板功能,安全剂量下咖啡因没有显示以上作用。Objective To investigate whether the consumption of coffee affects platelets function. Methods Twelve healthy men were enrolled in a 3-week crossover study. In 3 different sessions, subjects drank either coffee（containing 180 mg caffeine）, or a caffeine tablet（180mg）, or placebo. Platelet glycoproteins IIb/Ⅲa（GPIIb/Ⅲa）, and CD40 L expression were detected by flow cytometry at 0h, 2h, 4h. Effects of different concentrations of caffeine on platelet GPIIb/Ⅲa were detected in vitro. Results Coffee drinking inhibited ADP induced GPIIb/Ⅲa（P0.05 VS control at 2h and 4h） and CD40 L expression（P0.05 VS control at 2h and 4h）. Coffee drinking also inhibited arachidonic acid induced GPIIb/Ⅲa（P0.05 VS control at 2h and 4h） and CD40 L expression（P0.05 VS control at 2h）. Caffeine intake did not affect platelet GPIIb/Ⅲa, CD40 L expression induced by the agonists. Conclusion These results indicate that coffee is active in inhibiting platelet GPIIb/Ⅲa and CD40 L expression to some extent in vivo, and may be responsible for the beneficial effects of platelet function. Caffeine dose not show such effect at safe concentrations.

关 键 词：咖啡 咖啡因 血小板 动脉粥样硬化 

分 类 号：R151[医药卫生—营养与食品卫生学]