紫外诱变筛选高蛋白酶活米曲霉的研究  

Study on Screening of Aspergillus oryzae with High Proteinase Activity by UV Mutagenesis

作　　者：舒冬梅[1] 王德良[2] 宋绪磊[2] 尚柯[1] 

机构地区：[1]新疆农业大学食品科学与药学学院,乌鲁木齐830052 [2]中国食品发酵工业研究院,北京100015

出　　处：《中国调味品》2016年第3期81-84,94,共5页China Condiment

基　　金：科技部科研院所基金项目(2014EG111217)

摘　　要：为了提高米曲霉的蛋白酶活力,采用实验室保藏菌种蛋白酶活力较高的米曲霉菌株WT415为出发菌株,对米曲霉WT415采用紫外诱变,诱变条件：紫外灯功率40 W;距离35cm;诱变时间0,10,20,30,40,50,70,90,110,130,150s。试验结果表明：40 W紫外灯,35cm照射,90~110s时致死率为72.89%~81.32%,此时间为最佳照射时间;经紫外照射诱变后,米曲霉XWT4的蛋白酶活提高：酸性蛋白酶活提高14.87%,中性蛋白酶活提高48.85%,碱性蛋白酶活提高21.47%。并且经过10代传代培养,米曲霉XWT4具有稳定的遗传性状。To increase the proteinase activity of Aspergillus oryzae,take Aspergillus oryzae WT415 from laboratory as parent strain,and then adopt UV mutagenesis on it.The conditions are follows：UV light power is 40 W,distance is 35 cm,mutagenesis time is 0,10,20,30,40,50,70,90,110,130,150 s.The results show that 40 W UV irradiating for 90~110swith 35 cm distance is proper,under such conditions,the lethality is 72.89%~81.32%.Through UV irradiated mutagenesis,the acidic proteinase activity increases by 14.87%,neutral proteinase activity increases by 48.85%,alkaline proteinase activity increases by 21.47%.The hereditary characters of the mutant strain are very stable after 10generation＇s culture.

关 键 词：米曲霉 紫外诱变 蛋白酶活 遗传稳定性 

分 类 号：TS201.25[轻工技术与工程—食品科学]