纤维素酶辅助提取桑叶中叶蛋白的工艺  

Study on Cellulase Assisted Extraction Process of Leaf Protein from Morus Alba Leaves

作　　者：朱天明[1] 陈泠伶[1] 杨潇[1] 杨文宇[1] 陈祥贵[1] 

机构地区：[1]西华大学食品与生物工程学院,四川成都610039

出　　处：《西华大学学报（自然科学版）》2016年第2期77-81,109,共6页Journal of Xihua University:Natural Science Edition

基　　金：成都市企业自主创新一般项目计划(12GGYB755NC-001)

摘　　要：以桑叶为原料采用纤维素酶辅助提取叶蛋白。通过单因素实验分别考察固液比、加酶量、酶解时间对桑叶叶蛋白溶出量的影响。运用响应面分析方法,得到叶蛋白溶出量的二次多项式回归方程的预测模型,并预测和验证纤维素酶辅助提取桑叶叶蛋白最佳工艺条件为:加酶量为4%,提取时间为2 h,固液比1∶38(g/m L)。在此条件下,5 g桑叶叶蛋白溶出量为49.056 mg。The aim of this research is to extract leaf protein concentrates（ LPC） in Morus Alba leaves by cellulase enzyme. In single-factor experiment,the effect of ratio of solid-liquid,the amount of enzyme and extracting time on the dissolution rate LPC were investigated. The polynomial quadratic equations model was obtained by simulating the response surface analysis theory. At the same time,the optimum condition for the extraction of LPC in Morus Alba leaves assisted by cellulase enzymes is fixed. The amount of enzyme is 4%; the extraction duration is 2 hours; and the solid-liquid ratio is 1∶ 38（ g： m L）. In this condition,49. 056 LPC can be dissolved from 5g Morus Alba Leaves.

关 键 词：桑叶叶蛋白 纤维素酶 响应面法 工艺参数 

分 类 号：S727.3[农业科学—林木遗传育种]