茶多酚和葡萄籽提取物对假单胞菌抗生物被膜的抑制作用  

Inhibition Activity of Tea Polyphenols and Grape Seed Extract on Biofilm Formation of Pseudomonas

作　　者：朱军莉 王慧敏 王桂洋 陆海霞 励建荣[2] 

机构地区：[1]浙江工商大学食品与生物工程学院浙江省食品安全重点实验室,杭州310018 [2]渤海大学辽宁省食品安全重点实验室,辽宁锦州121013

出　　处：《中国食品学报》2016年第1期84-90,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31271954);浙江省自然科学基金(LY15C200001);浙江省科技厅项目(2012C22049)

摘　　要：研究茶多酚（TP）和葡萄籽提取物（GSE）对假单胞菌生物被膜形成的影响。采用结晶紫法、XTT法和银染法评价TP和GSE在不同质量浓度和不同添加时间下对假单胞菌抗生物被膜的作用。结果表明,2株铜绿假单胞菌能够形成被膜,其中1-3 d是快速形成期。随着作用质量浓度增加,TP和GSE对假单胞菌生长和生物被膜的抑制作用增强（P〈0.05）,其MIC分别为1 mg/mL和2 mg/mL,在亚抑菌浓度仍表现明显的抗生物被膜作用（P〈0.05）。1/2 MIC TP和GSE在被膜形成初期的12 h添加能显著抑制被膜形成（P〈0.05）,其中0 h添加效果最佳,抑制率为55%-67%;24 h后添加作用微弱,且TP和GSE不能清除成熟的生物被膜。TP和GSE处理组显著降低被膜菌的菌体活力（P〈0.05）,并且处理组细菌胞外分泌物低于对照组。TP和GSE在亚抑菌浓度下表现出较强的抗铜绿假单胞菌生物被膜作用,主要通过影响假单胞菌初期的粘附。本研究为植物多酚在食品贮藏和保鲜的广泛应用提供理论基础。The effect of tea polyphenols（TP） and grape seed extract（GSE） on biofilm formation of Pseudomonas was tested. Inhibitory activity of TP and GSE in the different concentration and different addition time on the biofilm formation of Pseudomonas aeruginosa were analyzed by crystal violet staining assay, XTT and sliver staining methods. The results showed that P. aeruginosa had ability of biofilm formation, in which 2-3 days were fast biofilm-forming period.Inhibition of growth and biofilm formation against P. aeruginosa gradually increased（P〈0.05） with the concentration increase of TP and GSE. The MIC of TP and GSE against P. aeruginosa was 1 mg/mL and 2 mg/mL respectively, and sub-MIC of TP and GSE exhibited strong inhibition of the biofilm formation. The addition of 1/2 MIC TP and GSE could significantly repress biolfilm formation（P〈0.05） during the initia 12 h, especially at 0 h the inhibition rate reaching 55-67%, whereas weak inhibitory effect was found after 24 h addition. No effect of TP and GSE on the matured biofilm was observed. Metabolic activity of the biofilms decreased considerably after incubation with TP and GSE. Biofilm matrices reduced in the treatment of TP and GSE compared with control. Therefore, TP and GSE at sub-MIC concentration exhibited strong resistance to biofilm of P. aeruginosa by inhibiting its initial adherence. The results offered a theoretical basis to wide application of plant polyphenols in food storage and preservation.

关 键 词：假单胞菌 生物被膜 茶多酚 葡萄籽提取物 抑制 

分 类 号：TS205[轻工技术与工程—食品科学]