毛细管区带电泳-间接紫外测定食品中木糖醇  

Determination of xylitol by capillary zone electrophoresis with indirect ultraviolet detection in food

作　　者：李疆[1] 陈桐[1,2,3] 鞠昭函 赵珊[1] 丁晓静[1,3] 

机构地区：[1]北京市疾病预防控制中心,食物中毒诊断溯源技术北京市重点实验室,北京100013 [2]首都医科大学附属北京安贞医院,北京100029 [3]首都医科大学公共卫生学院,北京100069 [4]北京联合大学应用文理学院,北京100083

出　　处：《食品安全质量检测学报》2016年第2期791-797,共7页Journal of Food Safety and Quality

基　　金：北京市卫生系统高层次卫生技术人才培养项目(2013-2-036)~~

摘　　要：目的建立毛细管区带电泳间接紫外测定食品中木糖醇的新方法。方法样品用超纯水提取后离心,过0.45μm膜后上机进样。以未涂敷熔融石英毛细管（50μm×60.2 cm）为毛细管分离柱,以8 mmol/L 3,5-二硝基苯甲酸、10 mmol/L硼砂和0.5 mmol/L十六烷基三甲基溴化铵为分离缓冲溶液。分离电压为-20 k V,检测波长为200 nm,用外标法定量。结果方法检出限为3.00 mg/L（S/N=3）,定量限为10.00 mg/L（S/N=9）,线性范围为10.00~300.0 mg/L,线性相关系数r为0.9994。在20.00、40.00、80.00 mg/L添加水平下,平均回收率分别为103.2%、103.3%及104.8%,相对标准偏差分别为0.5%、1.2%及0.7%（n=3）。结论该方法简单快速,11 min内即可完成一次样品分析（清洗6 min、分离5 min）,试剂及样品消耗量少,适用于食品中木糖醇的检测。Objective To develop a new method for the detection of xylitol in food by capillary zone electrophoresis with indirect ultraviolet. Methods Samples were extracted with ultrapure water, centrifuged and injected by 0.45 μm membrane. The separation was carried out using an uncoated fused-silica capillary with 50 μm i.d. and 60.2 cm total length. The separation buffer consisted of 8 mmol/L 3, 5-dinitrobenzoic acid, 10 mmol/L sodium tetraborate and 0.5 mmol/L hexadecyl trimethyl ammonium bromide, separation voltage was-20 k V and the detection wavelength was 200 nm. Quantification was made by external calibration between the corrected peak area and the concentration of xylitol. Results The limit of detection and limit of quantitation were 3.00 mg/L（S/N=3） and 10.00 mg/L（S/N=9）, respectively. The linear range between the corrected peak area and the concentration was from 10.00 to 300.0 mg/L with a correlation coefficient of 0.9994. The average spiked recoveries of 3 levels（20.00, 40.00 and 80.00 mg/L） were 103.2, 103.3 and 104.8% with relative standard deviations of 0.5%, 1.2% and 0.7%, respectively. Conclusion The method is simple and fast and the analysis could be completed within 11 min（6 min for rinsing and 5 min for separation）, which is suitable for the determination of xylitol in food samples.

关 键 词：毛细管区带电泳 间接紫外法 木糖醇 3 5-二硝基苯甲酸 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657[轻工技术与工程—食品科学与工程]