利用控pH流加培养法提高雨生红球藻的生物量及虾青素产量  

Enhancing biomass of Haematococcus pluvialis and astaxanthin production by pH-controlled fed-batch culture

作　　者：王旎[1] 管斌[1] 孔青[1] 孙翰[1] 耿兆艳 段良飞[1] 

机构地区：[1]中国海洋大学食品科学与工程学院

出　　处：《食品与发酵工业》2016年第2期120-124,共5页Food and Fermentation Industries

基　　金：国家"十二五"农村领域科技计划项目(2013BAD10B02-06);山东省科技发展计划项目(2014GSF121029);国家自然科学基金资助项目(31471657)

摘　　要：研究了一种新型培养方法——控pH流加培养法,可有效促进雨生红球藻生长并提高虾青素产量。测定雨生红球藻生长期间对营养盐的需求量,依据对数生长期藻液pH与硝酸盐和磷酸盐的含量的线性关系,确定流加液组成及流加方法。流加液的组成为7.88 g/L的KNO3,1.83 g/L的KH2PO4和24.0 g/L的CH3COONa,监测对数生长期藻液pH变化情况,通过添加流加液将藻液的pH值持续控制在7.5～8.0内,雨生红球藻的细胞干重可达（1.42±0.04）g/L,虾青素产量可达（60.23±1.81）mg/L。In this research,a new method of pH-controlled fed-batch culture was utilized to enhance dry cell weight of Haematococcus pluvialis and production of astaxanthin. Conditions of nutrient consumption of Haematococcus pluvialis were measured. The composition of fed-batch media and the details of fed-batch were determined based on the linear relationships between pH and consumption of nutrients of Haematococcus pluvialis in its logarithmic phase.The fed-batch media were consisted of 7. 88 g / L nitrate,1. 83 g / L phosphate and 24. 0 g / L sodium acetate. By monitoring and controlling pH of the suspension to the range of 7. 5 ～ 8. 0 with the fed-batch media in its logarithmic phase,the dry cell weight of Haematococcus pluvialis was enhanced to（ 1. 42 ± 0. 04） g / L,with the production of astaxanthin reaching（ 60. 23 ± 1. 81） mg / L.

关 键 词：雨生红球藻 虾青素 PH 流加培养 

分 类 号：TS264.4[轻工技术与工程—发酵工程]