杏鲍菇生产包黑斑病害菌分析  

Analysis of Disease Bacteria on Blackspot of Pleurotus eryngii Package

作　　者：姚璐晔[1] 沈金波[1] 裴丹丹[1] 孙丹凤[1] 冀宏[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《食品研究与开发》2016年第1期190-194,共5页Food Research and Development

基　　金：苏州应用基础研究项目(SYN201007;SYN201212)

摘　　要：杏鲍菇生产厂的部分菌丝生产包出现黑斑,从中分离纯化得到病害菌(130305S),结合显微结构和生理生化试验结果,以及对其16S rDNA序列的分析确定病害菌的系统发育地位;通过人工回染试验确定分离得到的病害菌与黑斑出现的相关性。结果表明:菌株130305S鉴定为巨大芽孢杆菌属(Bacillus megaterium);人工回染试验证实菌株130305S可导致生产包出现黑斑,菌丝不生长的污染症状。This study aimed to analysis the disease bacteria which caused blackspot in Pleurotus eryngii package. The strain named 130305 S was isolated from the polluted mushroom fungus package from Pleurotus eryngii factory. According to its microstructure, physiological, biochemical properties and 16 S rDNA sequences, the phylogenetic position of the strain was defined. The artificial infection experiment which affirmed the correlation between strain 130305 S and blacksport was also done. The results showed that 130305 S was identified as Bacillus megateriu. The phenomenon of blackspot appearance, that mycelium was no growth in package was caused by strain 130305 S.

关 键 词：杏鲍菇 黑斑 病害菌 巨大芽孢杆菌 人工回染 

分 类 号：S436.461[农业科学—农业昆虫与害虫防治]