动物双歧杆菌乳亚种BZ11的耐氧驯化及降胆固醇性能的研究  

Studies on oxygen domestication and cholesterol degradation property of Bifidobacterium animalis subsp. lactis BZ11

作　　者：王猛[1,2] 熊江[1,3] 张玲[1,2] 李翠芹[4] 何腊平[1,2,3] 陈平[5] 范劲[1] 

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [3]贵州省农畜产品贮藏与加工重点实验室,贵州贵阳550025 [4]贵州大学化学与化工学院,贵州贵阳550025 [5]贵州大学理学院,贵阳550025

出　　处：《食品与发酵工业》2016年第3期1-7,共7页Food and Fermentation Industries

基　　金：国家自然科学基金(31160002);贵州省猪肉制品工程技术研究中心项目(黔科合农G字[2013]4001号);贵州省猪肉特色制品深加工关键技术研究及产业化示范(黔科合重大专项字[2015]6004号)

摘　　要：为了提高动物双歧杆菌乳亚种BZ11对有氧环境的耐受性,采用逐渐增加培养基中的氧分压和有氧、厌氧条件下交替培养两种方案对其进行了耐氧驯化。通过对驯化前后菌株的生长特性进行分析得出逐渐增加培养基中的氧分压的驯化方案取得了更好的效果。经最优方案驯化后的菌株在有氧条件下培养20 h后,活菌数可达到6.30×10^8CFU/m L,是初始菌株的2.24倍。驯化后菌株的降胆固醇率为27.31%±0.80%,与初始菌株相比没有显著性差异（P〉0.05）。通过鉴定得出,动物双歧杆菌乳亚种BZ11在耐氧驯化前后保持了相似的形态及生理生化特征。因此,驯化后的菌株有望作为1株潜在益生菌被开发利用。To improve the aerotolerant capacity of Bifidobacterium animalis subsp. lactis BZ11 in aerobic environment,two ways including gradual increase of the partial pressure of oxygen in medium and alternate incubation between aerobic and anaerobic were taken. After analyzing the growth characteristics of the strain pre- and post- domestication,the first method obtained a better effect and cell concentration of the domesticated strain reached 6. 30 × 10^8 CFU/m L after cultivating in aerobic conditions for 20 h,which was 2. 24 times of the concentration of the original strain. The cholesterol degradation rate of the strain after oxygen-domestication was 27. 31% ± 0. 80%,which didn＇t show significant difference（ P〉0. 05） compared with the original strain. Moreover,identification results indicated that the physiological and morphological characteristics of domesticated Bifidobacterium animalis subsp. lactis BZ11 kept similar to those of the original strain. So it can be used as a potential probiotics for further in-depth development and utilization.

关 键 词：双歧杆菌 耐氧驯化 降胆固醇 鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]