双氢青蒿素对神经母细胞瘤细胞生长的抑制作用及其机制  

Inhibitory effect of dihydroartemisinin on growth of neuroblastoma cells and its mechanism

作　　者：齐玲[1] 杨阳[1] 刘玉翠[1] 朱天信 金嵩[1] 臧琳[1] 张玉影[1] 吕鹏[1] 徐冶[2] 

机构地区：[1]吉林医药学院病理教研室,吉林吉林132013 [2]吉林医药学院科研实验室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2016年第2期266-270,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81201671);吉林省科技厅科研基金资助课题(20140414049GH);吉林省卫生厅科研基金资助课题(2014Z104)

摘　　要：目的:探讨双氢青蒿素(DHA)对神经母细胞瘤细胞生长的抑制作用,阐明DHA抗神经母细胞瘤的作用机制。方法:实验分为空白对照组和DHA组(DHA终浓度分别为0.05、0.50、5.00和50.00μmol·L^(-1))。采用MTT法检测DHA作用后SH-SY5Y细胞的增殖率,流式细胞术分析DHA作用后SH-SY5Y细胞周期的变化,ELISA和Western blotting法分析DHA作用后SH-SY5Y细胞中cyclin D1和caspase-3蛋白表达水平的变化。结果:不同浓度DHA作用SH-SY5Y细胞24、48和72h时均可抑制细胞的生长,与空白对照组比较,0.50、5.00和50.00μmol·L^(-1) DHA组SH-SY5Y细胞增殖率明显下降(P<0.05或P<0.01);细胞生长的密度随药物浓度的升高而降低。与空白对照组比较,50.00μmol·L^(-1) DHA组SubG1期细胞的百分比增加(P<0.05);G0/G1期细胞百分比先升高再降低,S期与G2/M期细胞的百分比减少。与空白对照组比较,50.00μmol·L^(-1) DHA组SH-SY5Y细胞中cyclin D1蛋白表达水平降低(P<0.05);与空白对照组比较,50.00μmol·L^(-1) DHA组caspase-3蛋白表达水平升高(P<0.05)。结论:DHA可能通过阻滞肿瘤细胞周期、促进肿瘤细胞凋亡而抑制神经母细胞瘤细胞的生长。Objective： To explore the inhibitory effect of dihydroartemisinin （DHA） on the growth of neuroblastoma ceils, and to clarify the anti-tumor mechanism of DHA. Methods： The experiment was divided into blank control group and DHA groups （the final concentrations of DHA were 0.05, 0.50, 5.00 and 50. 00 μmol. L-1）. The proliferation rates of neuroblastoma SH-SY5Y cells after treated with DHA were examined by MTT assay; the changes of cell cycle of SH-SY5Y cells after treated with DHA were examined by flow cytometry; the expression levels of cyelin D1 and caspase-3 proteins were detected by ELISA and Western blotting methods. Results： The proliferation of SH-SY5Y ceils 24, 48, and 72 h after treated with different concentrations of DHA were inhibited. Compared with blank control group, the proliferation rates of SH-SY5Y cells in 0.50, 5.00 and 50.00 /xmol ~ L-1 DHA groups were significantly decreased （P〈0.05 or P〈0.01）. The density of cells was decreased with the increasing of DHA concentration. Compared with blank control group, the percentage of SH- SY5Y cells at SubG1 phase in 50.00/,mol · g-1 DHA group was increased （P（0.05）, and the percentage of cells at G0/G1 phase was increased first then was decreased~ otherwise, the percentages of cells at S and G2/M phase were decreased. Compared with blank control group, the expression level of cyclin D1 protein in 50.00 μmol · L-1 DHA group was decreased （P〈0.05）, but the expression level of caspase-3 protein in 50.00 tzmol · L-1 DHA group was increased （P〈0.05）. Oonclusion： DHA could inhibit the proliferation through arresting the cell cycle and inducing the apoptosis of neuroblastoma cells.

关 键 词：双氢青蒿素 神经母细胞瘤 CYCLIN D1 caspase-3 

分 类 号：R739.4[医药卫生—肿瘤]