响应面试验优化“Amano”β-糖苷酶水解槐角异黄酮工艺  

Process Optimization by Response Surface Methodology for Hydrolysis of Fructus Sophorae Isoflavones by “Amano” β-Glucosidase

作　　者：张晓松[1] 张博雅[1] 张永忠[1] 金花[1] 

机构地区：[1]东北农业大学理学院,黑龙江哈尔滨150030

出　　处：《食品科学》2016年第6期82-88,共7页Food Science

摘　　要：利用"Amano"β-糖苷酶对槐角异黄酮进行水解制备染料木黄酮。采用高效液相色谱法检测槐角异黄酮水解率。探讨酶质量浓度、水解时间、p H值和水解温度对异黄酮水解率的影响,并通过响应面法确定最佳水解条件。结果表明,最佳水解条件为酶质量浓度0.5 mg/m L、水解时间3.68 h、p H 4.95、水解温度59.2℃。在此条件下,槐角异黄酮水解率达95.76%。该研究为自然界药用植物的开发应用提供了理论依据。Purpose： To optimize the hydrolysis（into genistein） of isoflavones extracted from Fructus Sophorae with ＂Amano＂β-glucosidase. Methods： The optimization of four hydrolysis conditions including enzyme concentration, time, p H and temperature for improved hydrolysis efficiency was performed using response surface methodology（RSM）. Results： The optimal hydrolysis conditions were determined as follows： enzyme concentration, 0.5 mg/m L; hydrolysis time, 3.68 h; p H value, 4.95, and hydrolysis temperature, 59.2 ℃. Under these conditions, the hydrolysis efficiency of total isoflavones was as high as 95.76%. Our investigation is significant for the exploitation and application of medicinal pants.

关 键 词：槐角异黄酮 酶水解 高效液相色谱 

分 类 号：Q946.8[生物学—植物学]