玉米秸秆酶水解过程中的酶复配条件优化  

The Optimization of Enzyme Complex Formulation for Enzymatic Hydrolysis of Corn Stalk

作　　者：王奇[1] 王林风[1] 闫德冉[1] 张斐洋 刘天天[1] 吴静波[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,南阳473000

出　　处：《生物技术通报》2016年第3期171-177,共7页Biotechnology Bulletin

基　　金：国家科技支撑计划(2012BAC18B03);车用生物燃料技术国家重点实验室开放基金(2013030)

摘　　要：降低酶解成本是纤维素乙醇生产的关键。利用酶复配技术优化蒸汽爆破处理后玉米秸秆的酶水解工艺条件,以提高纤维素的转化率。通过单因素实验和正交实验,研究了纤维素酶、木聚糖酶和β-葡萄糖苷酶对酶解效率的影响规律。结果表明,汽爆玉米秸秆,纤维素含量达42.21%,半纤维素仅为3.65%。纤维素酶对酶解过程起决定性作用,添加40 FPU/g时,酶解率为75.45%;木聚糖酶可促使更多的纤维素暴露出来,添加1 500 IU/g时,酶解率最高为78.03%;β-葡萄糖苷酶有助于消除纤维二糖积累造成的反馈抑制,用量40 IU/g时,纤维二糖浓度为0.330 4 g/100 m L,酶解率达76.45%。正交实验确定最佳工艺为:纤维素酶用量30 FPU/g,木聚糖酶用量800 IU/g,β-葡萄糖苷酶用量40 IU/g;该条件下,进行底物质量浓度25%的验证实验,葡萄糖达9.3g/100 m L,若用单一天冠纤维素酶,葡萄糖仅5.9 g/100 m L,提高了57.63%。三种酶的影响顺序为:纤维素酶>木聚糖酶>β-葡萄糖苷酶。Reducing the cost of enzymatic hydrolysis is the key step in bio-ethanol production from cellulose. In order to enhance the conversion of cellulose into ethanol,the enzymatic hydrolysis condition of corn stalk pretreated by steam explosion was optimized by enzyme complex formulation. The affecting rules of cellulase,xylanase and β-glycosidase on enzymatic hydrolysis efficiency were studied by singlefactor experiment and orthogonal test. The results showed that the cellulose content of steam-exploded corn stalk reached 42.21%,and the hemicellulose content was only 3.65%. Cellulase was crucial to the enzymatic hydrolysis process,the enzymatic hydrolyzation yield was 75.45% when added by 40 FPU/g. Xylanase promoted more cellulose exposed,enzymatic hydrolyzation yield reached 78.03% when added by 1 500 IU/g. β-glucosidase was conducive to eliminate the feedback inhibition caused by cellobiose,when dosage was in 40 IU/g,the concentration of cellobiose was 0.330 4 g/100 m L,enzymatic hydrolyzation yield reached 76.45%. The optimal conditions for the process by orthogonal experiment were as follows ：cellulase 30 FPU/g,xylanase 800 IU/g,and β-glucosidase 40 IU/g,under this and when the substrate concentration was 25%,glucose was up to 9.3 g/100 m L,i.e.,increased by 57.63% compared to using a single cellulase while the glucose was only 5.9 g/100 m L. The affecting significance of three enzymes was ordered as follows ：cellulase xylanase β-glucosidase.

关 键 词：玉米秸秆 纤维素酶 木聚糖酶 Β-葡萄糖苷酶 复合配方 

分 类 号：S216.2[农业科学—农业机械化工程]