槟榔花中酚类物质对HSF细胞氧化损伤的保护作用  

Protective Effects of Phenolic Compounds in Areca Inflorescence on Oxidative Damage in HSF Cells

作　　者：宋菲[1] 王齐齐[2] 王挥[1] 黄玉林[1] 陈卫军[1] 赵松林[1] 

机构地区：[1]中国热带农业科学院椰子研究所,海南文昌571339 [2]海南大学食品学院,海南海口570228

出　　处：《食品研究与开发》2015年第23期10-13,46,共5页Food Research and Development

基　　金：海南省自然科学基金(314146)

摘　　要：为了探讨槟榔花中3种主要酚类物质（表儿茶素、没食子酸、香豆酸）对人皮肤成纤维细胞（HSF）氧化损伤的保护作用,以H_2O_2（150μmol/L）处理HSF细胞24 h,建立氧化损伤模型,MTT法测定细胞存活率分光光度法检测细胞内总抗氧化能力和线粒体膜肿胀度,DCFH-DA染色法测定细胞内活性氧的含量。结果表明表儿茶素和没食子酸均能改善H_2O_2引起的HSF细胞的氧化损伤而香豆酸却没有保护作用。与H_2O_2模型组相比表儿茶素和没食子酸均能提高氧化损伤细胞的存活率,并将细胞内总抗氧化能力提高了4.73%-31.66%,细胞内活性氧和线粒体膜肿胀度也明显降低。表明槟榔花中2种主要的酚类物质表儿茶素和没食子酸对HSF细胞氧化损伤具有保护作用。In order to understand the protective effects of 3 kinds of phenolic compounds（epicatechin,gallic acid,coumaric acid） in Areca inflorescence on oxidative damage in human skin fibroblasts cells（HSF）,HSF cells were treated with 150 μmol/L H_2O_2 for 24 h and the oxidative damage model was established.Cell viability was measured by MTT method,the total antioxidant capacity and mitochondria membrane swelling degree were detected by spectrophotometry,intracellular reactive oxygen species（ROS）accumulation was measured by DCFH-DA staining method.The results showed that epicatechin and gallic acid could improve the oxidative damage on HSF cells induced by H_2O_2,whereas coumaric acid had no protective effect.Compared with the H_2O_2model group,epicatechin and gallic acid can improve the survival rate of HSF cells,the total antioxidant capacity was increased by 4.73%-31.66%,intracellular reactive oxygen species and the mitochondrial swelling decreased.As a results,the two main kinds of phenolic compounds（epicatechin and gallic acid） in Areca inflorescence have protective effects on oxidative damage in HSF cells.

关 键 词：槟榔花 酚类物质 HSF细胞 氧化损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]