分离的巴马人群肠道乳酸菌数量及功能性分析  

Analyze the Amount of Lactic Acid Bacteria from Population of Bama and Their Function

作　　者：郭飞翔[1] 陈大卫[1] 石雪晨 赵海晴 伍云 黄嘉棣 顾瑞霞[1] 

机构地区：[1]江苏省乳品生物技术与安全控制重点实验室扬州大学食品科学与工程学院,江苏扬州225127 [2]广西皇氏甲天下乳业股份有限公司,广西南宁530007

出　　处：《食品研究与开发》2015年第24期164-167,200,共5页Food Research and Development

基　　金：江苏高校优势学科建设工程项目;"十一五"国家科技支撑计划项目(2013BAD18B12);江苏省科技支撑项目(BE2011383);江苏省高校自然科学研究重大项目(12KJA550003)

摘　　要：对采集的广西巴马地区长寿家族粪便样品进行菌株分离,通过平板计数,比浊法和平板扩散法对分离的乳酸菌菌群数量、耐受力和抑菌能力分析。结果表明:不同分离培养基之间的菌落总数有差异,并且杆菌选择性培养基菌落数(108 cfu/g^109 cfu/g)要多于球菌选择性培养基菌落数(107 cfu/g^108 cfu/g);不同性别之间分离的女性肠道乳酸菌总数(8.98±0.42)(lg cfu/g)略高于男性(8.87±0.46)(lg cfu/g),不同年龄组中青少年组的肠道乳酸菌数量最多;分离的乳酸菌耐受能力比较发现中年组(0.408 5±0.082 7)(lg cfu/g)与老年组(0.372 6±0.083 2)(lg cfu/g)的耐受胃液能力相当,且显著大于青少年组(0.339 5±0.072 5)(lg cfu/g)和长寿组(0.282 6±0.038 5)(lg cfu/g);不同年龄组的耐胆盐能力和耐受肠液能力相当;分离的乳酸菌抑菌能力比较发现分离的不同年龄组的乳酸菌抑制大肠杆菌、金黄色葡萄球菌能力都是青少年组最强,同一年龄组的乳酸菌抑制大肠杆菌能力较强;分离的男性肠道内的乳酸菌抑制大肠杆菌能力要低于女性,但抑制金黄色葡萄球菌能力相当。Collect the stool specimens from longevity village population of Bama for separating strain and analyzing the amount of them and capacity of tolerance and inhibition by plate colony-counting, turbidimetry,plate diffusion. The results showed that： the amount of colony between different isolation medium had difference, and the amount of bacillus（108cfu/g-109cfu/g） was more than coccus（107cfu/g-108cfu/g）; the amount of colony from female（8.98±0.42）（lg cfu/g） was more than male（8.87±0.46）（lg cfu/g）, youth group had was the most among age groups; the capacity of tolerating gastric juice of middle age group（0.408 5±0.082 7）（lg cfu/g） and old age group（0.372 6±0.083 2）（lg cfu/g） were similar, and theirs were significantly higher than youth（0.339 5±0.072 5）（lg cfu/g） and longevity group（0.282 6±0.038 5）（lg cfu/g）; capacity of tolerating bile and intestinal juice between age groups had no significant difference; the youth group had the strongest inhibitory effect on E.coil and Staphylococcus aureus among age groups, and the strains had stronger inhibitory effect on E.coil than Staphylococcus aureus; the strain from male had lower inhibitory effect on E.coil than female, but had no significant difference on Staphylococcus aureus.

关 键 词：长寿 肠道 乳酸菌 耐受性 抑菌 

分 类 号：R37[医药卫生—病原生物学]