DHA/EPA摄入比例对小鼠氧化应激和炎症的影响  

Effects of the DHA/EPA ratios on oxidative stress and inflammatory factors in mice

作　　者：刘亮[1] 胡勤玲[1] 薛伊宏 蔡亮[1] 吴辉辉[1] 吴永宁[1] 宫智勇[1] 

机构地区：[1]武汉轻工大学食品科学与工程学院农产品加工湖北省协同创新中心,武汉430023

出　　处：《食品科技》2016年第3期105-110,共6页Food Science and Technology

基　　金：国家自然科学基金青年基金项目(31201351);湖北省自然科学基金项目(2013CFB481)

摘　　要：目的:研究DHA/EPA比例对高脂膳食小鼠肝脏氧化应激和炎症因子表达的影响。方法:将40只雄性apo E-/-小鼠随机分为4组并给予高脂膳食,同时用葵花籽油、紫苏油、鱼油和藻油等调节膳食中n6/n3 PUFA的比例为4:1或DHA/EPA的比例(2:1、1:1和1:2)。12周后,用商业试剂盒或实时荧光定量PCR法检测小鼠血清或肝脏组织的氧化应激水平或炎症因子表达变化。结果:DHA/EPA替代植物来源的n3 PUFA-ALA并不能减少血清脂质过氧化产物MDA生成及提高血清和肝脏的SOD活性;与补充ALA相比,DHA/EPA为1:1时小鼠血清GSH含量显著升高(P<0.05),DHA/EPA比例为1:1和1:2时小鼠肝脏的GSH水平显著升高(P<0.05);DHA/EPA比例2:1可显著降低血清及肝脏的促炎因子表达。结论:在高脂膳食及膳食n6/n3 PUFA比例4:1的条件下,补充DHA/EPA比植物来源的ALA更能提高抗氧化能力和降低炎症水平,且当DHA/EPA比例1:1时抗氧化能力最好,而当DHA/EPA比例2:1时抗炎能力最强。Objective：To investigate the ratios of DHA/EPA on oxidative stress and inflammatory factors in mice treated with high-fat diets.Methods：Forty male apo E-/-mice were randomly divided into 4 groups and were given high-fat diets.Meanwhile,sunflower seed,perilla,fish,and algal oils were used for adjusting the proportion of n6/n3 PUFA 4：1 and DHA/EPA（2：1,1：1,and 1：2）.After 12 weeks feeding,commercial kits and/or real-time PCR were used to test the redox status and inflammatory factors expression in mice serum and/or liver.Resuts：Replacement of ALA with DHA and EPA could not decreaseserum MDA production and improve serum and hepatic SOD activity.Compare to ALA group,serum GSH levels were increased significantly（P〈0.05） in DHA/EPA 1：1 group.The levels of GSH in serum and liver were both improved（P〈0.05） in DHA/EPA 1：1 and 1：2 groups.The expression of pro-inflammatory factors in serum and liver was reduced（P〈0.05） in DHA/EPA 2：1 group.Conclusion：Supplementation with DHA/EPA was superior to ALA when consumed high-fat diets with n6/n3 PUFA ratio 4：1,since the antioxidant ability of DHA/EPA 1：1 and the anti-inflammatory capacity of DHA/EPA 2：1 are more powerful than ALA.

关 键 词：n3多不饱和脂肪酸 DHA EPA 炎症因子 氧化应激 

分 类 号：TS201.2[轻工技术与工程—食品科学]