豆浆中胰蛋白酶抑制剂活性测定方法的研究  

Determination of trypsin inhibitor activity of soybean milk

作　　者：殷帅[1,2] 姜成君 田洪[1] 孟庆玉[1] 李健和[3] 

机构地区：[1]中南大学药学院,长沙410013 [2]湖南省食品药品检验研究院,长沙410001 [3]中南大学湘雅二医院药学部,长沙410011

出　　处：《食品安全质量检测学报》2016年第3期1000-1005,共6页Journal of Food Safety and Quality

摘　　要：目的在GB/T 21498-2008《大豆制品中胰蛋白酶抑制剂活性的测定》方法的基础上,对影响豆浆中胰蛋白酶抑制剂活性测定的一些关键因素进行研究和改进。方法豆浆样品加水提取并稀释至一定体积后,加入L-BAPA试剂和胰蛋白酶使用液,(37±0.25)℃水浴中保温10 min±5 s后,加入乙酸溶液终止酶反应,并采用分光光度计在410 nm波长下测定其吸光度值,代入公式计算胰蛋白酶抑制剂的活性。结果改进后的方法添加高、中、低3个浓度水平胰蛋白酶抑制剂的回收率分别为113.1%、114.6%、134.9%,平行测定的RSD分别为1.4%、3.6%、7.2%,方法的准确性和重复性能满足实验的需求。结论改进后的方法快速简便,节约了时间和实验成本,方法的准确性和重复性能满足实验的需求。Objective To study and improve some key factors influencing determination of trypsin inhibitor activity of soybean milk,based on the method of GB/T 21498-2008 Determination of trypsin inhibitor activity of soya products.Methods Soybean milk samples were extracted by water and diluted to a certain volume,with adding L-BAPA reagent and trypsin using liquid,and（37＋0.25） ℃ water bath insulation for 10min±5 s,then the enzyme reaction was ceased by adding acetic acid.The absorbance value was determined at the wavelength of 410 nm by spectrophotometer and the trypsin inhibitor activity was calculated by substituting into the formula.Results The average recoveries of low,medium and high concentrations of standard samples were 113.1%,114.6%and 134.9%,and the RSDs of parallel determination were 1.4%,3.6%and 7.2%,respectively.The accuracy and reproducibility of the method has met the satisfaction of the experiment.Conclusions The improved method is time-saving and low-cost,and had high accuracy and precision,which also meets the requirements of the experiment.

关 键 词：豆浆 胰蛋白酶抑制剂活性 测定方法 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]