蛋白橘皮果胶胶体制备、表征及对红景天苷体外释放研究  

Research on preparation,characterization of bovine serum albumin and citrus peel pectin hydrogel and release for salidroside in vitro

作　　者：陈莎[1] 彭海龙[1,2] 胡振瀛[1] 宁方建 余宁翔 朱雯婷[1] 刘文强[1] 熊华[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学化学工程系,江西南昌330031

出　　处：《食品工业科技》2016年第8期139-144,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(21201098);江西省自然科学基金(20151BAB203029)

摘　　要：以牛血清蛋白与橘皮果胶为原料,采用安全无毒的自组装技术制备缓释水凝胶,并以制备过程中两种原料配比、蛋白浓度与p H进行三因素三水平对凝胶粒径影响的响应面分析。在此基础上,重点研究显著因子添加橘皮果胶比例对蛋白成胶的影响,并结合利用流变、圆二、扫描电镜对其进行表征。以红景天苷为模型检测其包封效果和体外释放,结果表明:橘皮果胶和牛血清蛋白比例为0.5和p H为4.5时加热粒径达到最小259.5 nm,尺寸分布较窄(PDI<0.06),形成良好稳定的三维网络结构水凝胶;红景天苷包封率达到49.88%,在模拟胃肠溶液中具有缓释效果。本研究的水凝胶可作为红景天苷以及其他功能性食品的潜在输送载体。A novel slow release hydrogel was prepared by bovine serum albumin（BSA） and citrus peel pectin via self-assembly. The significant influence on the diameter of hydrogel was found and discussed by three factors Mass ratio（MR） of pectin and BSA,protein concentration,and p H at three level in the preparation of the hydrogel. Combined with the characterization of rheology,CD and SEM,the paper further focused on investigating the effective factor MR of CPP and BSA. The results showed that mixture of pectin and BSA reached the smallest particle size 259.5 nm with a narrow size distribution（PDI0.06） and formed a good and stable hydrogel with three-dimensional network structure when heated with mass ratio（MR） of CPP：BSA 0.5 at p H4.5. The result indicated that the hydrogels（BCH） had a high encapsulation efficiency（EE,49.88%） and slow release under simulated gastric and intestinal fluid condition when salidroside was used as a model molecule,which showed that this novel hydrogel might be a potential deliver carrier for salidroside or other functional food agents.

关 键 词：牛血清蛋白 橘皮果胶 自组装 水凝胶 缓释 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]