Specific Electrochemiluminescence of Aptamer-Functionalized Quantum Dots with Lysozyme and Hemin as Co-Triggers  

作　　者：Xubo Ji Chuanguang Yao Ying Wan Hongxin Song Peng Xin Hongda Cui Chenyu Zheng Shengyuan Deng 

机构地区：[1]School of Environmental and Biological and Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China [2]School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China [3]State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing, Jiangsu 210093, China

出　　处：《Chinese Journal of Chemistry》2016年第3期331-336,共6页中国化学（英文版）

摘　　要：This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence （ECL） strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin-film as substrate to adsorb thiolated captures： a hairpin-structured DNA, integrating dual head-tail connected functional domains： one aptamer sequence for lysozyme and the other for hemin （iron（II1） proto-porphyrin IX）. In the presence of the both, the hairpin conformation unfolded and transformed into the hemird G-quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consump- tion of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 μg.mL-1 to 5μg. mL-1, with a detection limit as low as 4.95 pgo mL-1 （e.g. 9.4 pmol·L^-1）, dem- onstrating the prospective utilization of DNA technologies in bioanalysis.This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence （ECL） strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin-film as substrate to adsorb thiolated captures： a hairpin-structured DNA, integrating dual head-tail connected functional domains： one aptamer sequence for lysozyme and the other for hemin （iron（II1） proto-porphyrin IX）. In the presence of the both, the hairpin conformation unfolded and transformed into the hemird G-quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consump- tion of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 μg.mL-1 to 5μg. mL-1, with a detection limit as low as 4.95 pgo mL-1 （e.g. 9.4 pmol·L^-1）, dem- onstrating the prospective utilization of DNA technologies in bioanalysis.

关 键 词：APTASENSOR LYSOZYME G-QUADRUPLEX HEMIN ELECTROCHEMILUMINESCENCE 

分 类 号：O471.1[理学—半导体物理] Q554.6[理学—物理]