湘西香醋对秀丽隐杆线虫体内抗氧化作用  

In Vivo Antioxidant Activity of Xiangxi Vinegar: A Study in Caenorhabditis elegans

作　　者：黄润庭 李宗军[1] 谭雅[1] 杜莎[1] 吴硕[1] 伍婧[1] 

机构地区：[1]湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,湖南长沙410128

出　　处：《食品科学》2016年第7期208-212,共5页Food Science

摘　　要：本研究对秀丽隐杆线虫分别饲喂配制白醋、酿造白醋、发酵1 a香醋以及湘西原香醋,通过测定其体内活性氧(reactive oxygen species,ROS)含量,谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活力以及细胞凋亡相关蛋白表达情况,对湘西香醋体内抗氧化作用进行评价。结果表明:湘西香醋具有明显抗氧化效果,与对照组相比,发酵1 a香醋组与湘西原香醋组秀丽隐杆线虫体内ROS含量明显降低(P<0.01),分别为对照组的24.03%、39.44%。3种抗氧化酶活力明显升高(P<0.01),湘西原香醋组秀丽隐杆线虫体内GSH-Px、SOD、CAT活力分别为对照组的2、1.3、3倍。发酵1 a香醋组与湘西原香醋组秀丽隐杆线虫体内凋亡蛋白CED-3表达量明显降低,分别为对照组的53.42%、73.39%,抗凋亡蛋白CED-9表达量显著升高,分别约为对照组的7倍和6倍(P<0.01)。湘西香醋在秀丽隐杆线虫体内具有较强抗氧化作用。The antioxidant activity of Xiangxi vinegar was evaluated by determining the level of reactive oxygen species（ROS）, the activities of glutathione peroxidase（GSH-Px）, superoxide dismutase（SOD） and catalase（CAT）, and cell apoptos is in Caenorhabditis el egans. The results showed that Xiangxi vinegar had a remarkable antioxidant effect in C. elegans. Compared with the control group（M9 buffer）, the level of ROS in Caenorhabditis elegans treated with one-year fermented, one-year aged Xiangxi vinegar and one-year fermented Xiangxi vinegar decreased significantly（P〈0.01） by 39.44% and 24.03%, respectively. The activities of GSH-Px, SOD and CAT in the first treatment group increased remarkably（P〈0.01） compared with the control group, showing a 2-, 1.3- and 3-fold increase, respectively. The expression level of CED-3 in both Xiangxi vinegar treatment groups dropped significantly to 53.42% and 73.39% of the control group, respectively, whereas the expression level of CED-9 in the treatment groups increased significantly（P〈0.01） 7 and 6 times, respectively. Therefore, Xiangxi vinegar has strong antioxidant activity in C. elegans.

关 键 词：醋 秀丽隐杆线虫 活性氧 抗氧化酶 细胞凋亡 

分 类 号：TS264.2[轻工技术与工程—发酵工程]